转基因复合抗虫耐除草剂玉米BFL4-1的分子特征及功能评价  

Molecular Characteristics and Functional Evaluation of Transgenic Maize BFL4-1 With Stacked Insect and Herbicide Resistance Traits

作　　者：邹俊杰 徐妙云[1] 张兰[1] 郑红艳 王磊[1,2] ZOU Junjie;XU Miaoyun;ZHANG Lan;ZHENG Hongyan;WANG Lei(Key Laboratory of Agricultural Genomics(Beijing),Ministry of Agriculture and Rural Affair,Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China;National Nanfan Research Institute(Sanya),Chinese Academy of Agricultural Sciences,Hainan Sanya 572024,China)

机构地区：[1]中国农业科学院生物技术研究所,农业农村部农业基因组学重点实验室(北京),北京100081 [2]三亚中国农业科学院国家南繁研究院,海南三亚572024

出　　处：《中国农业科技导报》2022年第1期31-37,共7页Journal of Agricultural Science and Technology

基　　金：国家转基因生物新品种培育重大专项(2018ZX08003-03B);三亚崖州湾科技城管理局2020年度科技计划项目(SKJC-2020-02-005)。

摘　　要：玉米螟和田间杂草严重影响我国玉米的生长发育,导致玉米产量和品质下降。培育具有抗虫、耐除草剂等复合性状的转基因玉米成为转基因玉米育种的一个趋势。将cry1Ab、cry1F和cp4epsps构建在同一载体上并利用农杆菌转化到玉米Hi-II中,通过与玉米自交系郑58进行回交转育,获得了具有郑58遗传背景并兼具抗虫和耐除草剂性状的转基因玉米株系BFL4-1。全基因组高通量测序和侧翼序列测序分析表明,BFL4-1中目的基因插入片段位于第3染色体基因间区,为单一插入位点。转录水平和蛋白水平检测结果表明,cry1Ab、cry1F和cp4epsps在不同组织中均能稳定表达。田间玉米螟接虫和喷施草甘膦试验表明,BFL4-1株系高抗玉米螟并且对草甘膦具有较好的耐受性,与对照相比在农艺性状方面无显著差异。由此表明,获得的BFL4-1为具有抗玉米螟和耐草甘膦除草剂复合性状的转基因玉米新材料。Ostrinia furnacalis(Asian corn borer) and weeds seriously affect maize growth,development and production in China. Transgenic stacking maizes with insect and herbicide resistance traits have become the main trend of transgenic maize breeding. cry1 Ab,cry1 F and cp4 epsps were introduced into maize Hi-II mediated by Agrobacteria. After backcrossing with maize inbred line Zheng 58 and then self-pollination,one transgenic line named BFL4-1 was obtained. Through whole genome sequencing and flanking sequence analysis,there was one insertion in BFL4-1 and the insertion fragment was located in the intergenic region of chromosome 3. In BFL4-1 plants,cry1 Ab,cry1 F and cp4 epsps were detected in different tissues on transcription and protein levels. Field experiments revealed that the BFL4-1 transgenic plants were highly resistant to Asian corn borer and more tolerant to glyphosate. There were no significant differences between BFL4-1 and the control(Zheng 58)on agronomic traits. Above results showed that BFL4-1 line could be served as a new maize transgenic line with stacked traits showing high resistance to insect and glyphosate.

关 键 词：转基因玉米 抗虫 耐除草剂 复合性状 分子特征 遗传稳定性 

分 类 号：S513[农业科学—作物学]