Chloroplast gene matK holds the barcodes for identification of Momordica(Cucurbitaceae) species from Indian subcontinent  

作　　者：Girme Aoudumbar Ramesh Deepu Mathew K.Joseph John V.Ravisankarc 

机构地区：[1]Centre for Plant Biotechnology and Molecular Biology,Kerala Agricultural University,Thrissur,Kerala State 680656,India [2]ICAR-National Bureau of Plant Genetic Resources,Regional Station,Thrissur,Kerala State 680656,India [3]Bioinformatics Centre,Kerala Agricultural University,Thrissur,Kerala State,680656,India

出　　处：《Horticultural Plant Journal》2022年第1期89-98,共10页园艺学报（英文版）

基　　金：Department of Biotechnology(DBT),Govt.of India,for the fellowship and financial assistancefor his M.Sc.(Ag.)in Plant Biotechnolo gy research work(Grant No.CPBMB/CoH/DBT-HRD/12)。

摘　　要：DNA barcoding is a supplementary tool in plant systematics,extensively used to resolve the species level controversies.This paper details the identification of DNA barcodes for seven species of Momordica,using the chloroplast gene mat K.Since the species M.cymbalaria has been confused as a member of the genus Luffa,26 accessions of Momordica belonging to seven Indian species and two accessions of Luffa acutangula were included in this study.Analysis of mat K sequences has yielded distinct barcodes in M.charantia var.charantia,M.subangulata subsp.renigera,M.cochinchinensis,M.balsamina,M.cymbalaria and also in Luffa acutangula.Evolutionary status of each species was reflected as nucleotide polymorphisms in each sequence.The wild species M.dioica and M.sahyadrica have yielded one barcode but failed to get differentiated.Further,this study provides conclusive proof that M.cymbalaria is a member of Momordica genus.The phylogram generated was successful to distinguish the monoecious species of this genus,M.charantia,M.balsamina and M.cymbalaria,from the dioecious species M.dioica,M.sahyadrica,M.subangulata subsp.renigera and M.cochinchinensis.Thus,mat K locus,by accumulating the evolutionary sequence variations,is proven efficient to differentiate the Momordica species and to reveal their relatedness.

关 键 词：CUCURBIT DNA barcoding LUFFA Phylogeny systematics 

分 类 号：S642.5[农业科学—蔬菜学]