环状RNA hsa_circ_0001445在绝经后骨质疏松症患者中的表达及作用机制  

Expression and the relative mechanisms of circular RNA hsa_circ_0001445 in patients with postmenopausal osteoporosis

作　　者：杨玉娇 刘萍 滕若凌 刘芬芬 丁怡[1] Yang Yujiao;Liu Ping;Teng Ruoling;Liu Fenfen;Ding Yi(Department of Geriatrics,the First People's Hospital of Changzhou,Changzhou 213000,China)

机构地区：[1]常州市第一人民医院老年医学科,213000

出　　处：《中华老年医学杂志》2022年第1期30-35,共6页Chinese Journal of Geriatrics

基　　金：2021年江苏省卫生健康委员会老年健康科研课题项目(LK2021040);2020年常州市卫健委重大科技项目(ZD202008)。

摘　　要：目的探讨环状RNA(circRNA)hsa_circ_0001445在绝经后骨质疏松症(PMOP)患者中的临床意义和调控机制。方法从临床收集的血液样品中提取RNA,使用实时荧光定量PCR(qRT-PCR)检测hsa_circ_0001445的表达。荧光素酶报告基因分析、RNA下拉实验研究了RNA之间的相互作用。结果与健康对照组相比,PMOP患者血浆hsa_circ_0001445表达下调(P<0.001)。血浆中hsa_circ_0001445以高灵敏度和特异性将PMOP患者与健康对照组区分开来,曲线下面积(AUC)为0.9654(95%CI0.9361~0.9947,P<0.001),灵敏度为94.0%,特异度为88.0%。Hsa_circ_0001445促进人骨髓间充质干细胞(hBMSCs)的成骨分化、抑制成脂分化(P<0.001)。Hsa_circ_0001445可以直接与miR-127-5p结合。结论血浆hsa_circ_0001445是PMOP的潜在诊断生物标志物,通过海绵化miR-127-5p,调节hBMSCs中成骨和成脂之间的平衡。Objective To investigate the clinical significance and regulatory molecular mechanism of the circular RNA(circRNA)hsa_circ_0001445 in postmenopausal osteoporosis(PMOP).Methods RNA was extracted from clinically collected blood samples,and the expression of circRNA hsa_circ_0001445 was detected by quantitative real-time PCR(qRT-PCR).Luciferase reporter gene analysis and RNA pull-down experiments were performed to investigate the interaction between two genes.Results Compared with healthy controls,the plasma circRNA hsa_circ_0001445 in PMOP patients was down regulated(P<0.001).The circRNA hsa_circ_0001445 distinguished PMOP patients from healthy controls with high sensitivity and specificity.The area under the ROC curve(AUC)was 0.9654(95%CI:0.9361-0.9947,P<0.001),and the sensitivity was 94.0%,while the specificity was 88.0%.The circRNA hsa_circ_0001445 promoted the osteogenic differentiation and inhibited the adipogenic differentiation of hBMSCs.The circRNA hsa_circ_0001445 can directly bind to miR-127-5p.Conclusions Plasma level of the circRNA hsa_circ_0001445 is a potential diagnostic biomarker of PMOP and regulates the balance between osteogenesis and adipogenesis in hBMSCs by sponging miR-127-5p.

关 键 词：骨质疏松 绝经后 氨基酸类 环状 间质干细胞 

分 类 号：R580[医药卫生—内分泌]