冬季不同海拔大绒鼠MC1R基因的表达量及UCP1、Hb、Mb含量的比较  

Comparative on the expression of MC1R gene and the contents of UCP1,Hb and Mb of Eothenomys miletus at different altitudes from Hengduan Mountain region in winter

作　　者：韩春艳 贾婷 王炎 王政昆[1] 朱万龙[1] 张浩[1] HAN Chunyan;JIA Ting;WANG Yan;WANG Zhengkun;ZHU Wanlong;ZHANG Hao(Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan,School of Life Sciences,Yunnan Normal University,Kunming 650500,China;Yunnan College of Business Management,Kunming 650106,China)

机构地区：[1]云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室,云南师范大学生命科学学院,昆明650500 [2]云南经济管理学院,昆明650106

出　　处：《兽类学报》2022年第1期69-75,共7页Acta Theriologica Sinica

基　　金：国家自然科学基金(31760118,32160254);云南省中青年学术和技术带头人后备人才项目(2019HB013);云南省万人计划青年拔尖人才项目(YNWR-QNRC-2019-047)。

摘　　要：为探究横断山冬季不同海拔大绒鼠的毛色差异、生理及血液指标的变化情况,自北向南从德钦、香格里拉、丽江、剑川和哀牢山5个地区按高海拔至低海拔进行采样,通过PCR扩增大绒鼠黑素皮质素受体1(melanocortin receptor 1,MC1R)基因序列,使用FQ-PCR测定其皮肤的MC1R基因表达量,并利用酶联免疫分析法(ELISA)测定褐色脂肪组织中解偶联蛋白1(uncoupling protein 1,UCP1)含量、血清中血红蛋白和肌红蛋白含量。结果表明:扩增出的MC1R基因片段为约200 bp的部分序列;哀牢山和剑川地区的大绒鼠MC1R基因表达量显著高于丽江、香格里拉和德钦地区;德钦和香格里拉地区的UCP1含量和肌红蛋白含量显著高于哀牢山、剑川和丽江地区;5个地区间血红蛋白含量差异不显著。以上结果表明大绒鼠通过改变MC1R基因的表达量来改变毛色,还通过升高肌红蛋白含量和UCP1含量来增加产热能力,从而适应横断山不同海拔的环境变化。In order to explore the variations of coat color,physiological and blood indexes in Eothenomys miletus at different elevations in winter,samples were collected from north to south of Hengduan Mountain region in Deqin(DQ),Shangri-la(XGLL),Lijiang(LJ),Jianchuan(JC)and Ailao Mountain(ALS)from north to south,Gene sequence of melanocortin receptor1(MC1R)was amplified by PCR and the expression of MC1R gene in skin was determined by FQPCR.The contents of uncoupling protein 1(UCP1)in brown adipose tissue,hemoglobin(Hb)and myoglobin(Mb)in serum were measured by enzyme-linked immunoassay(ELISA).The results showed that the amplified MC1R fragment was a partial sequence of about 200 bp.MC1R gene expression in E.miletus from ALS and JC was significantly higher than from LJ,XGLL and DQ.Contents of UCP1 and Mb in E.miletus from DQ and XGLL were significantly higher than that those from ALS,JC and LJ.But Hb content showed no significant difference among the five regions.All of the above results showed that E.miletus could change the coat color by changing the expression of MC1R gene and increase the thermogenesis capacity by elevating the content of Mb and UCP1 in order to adapt to the environmental changes at different altitudes from Hengduan Mountain region.

关 键 词：大绒鼠 毛色基因 UCP1 血红蛋白 肌红蛋白 

分 类 号：Q494[生物学—生理学]