植物染色体分子核型技术研究进展  

Research progress of molecular karyotype of plants

作　　者：刘青 潘浩研[1,3] 黄家丽 JOHN Seymour Heslop Harrison LIU Qing;PAN Haoyan;HUANG Jiali;JOHN Seymour Heslop HARRISON(Key Laboratory of Plant Resources Conservation and Sustainable Utilization,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,Guangdong 510650,China;Center for Conservation Biology,Core Botanical Gardens,Chinese Academy of Sciences,Guangzhou,Guangdong 510650,China;China University of Chinese Academy of Sciences,Beijing 100049,China;College of Life Sciences,South China Agricultural University,Guangzhou,Guangdong 510642,China;Department of Genetics and Genome Biology,University of Leicester,Leicester,UK LE17RH)

机构地区：[1]中国科学院华南植物园,植物资源保护与可持续利用重点实验室,广东广州510650 [2]中国科学院核心植物园,广东广州510650 [3]中国科学院大学,北京100049 [4]华南农业大学生命科学学院,广东广州510642 [5]莱斯特大学遗传学和基因组生物学系,英国莱斯特LE17RH

出　　处：《西北农林科技大学学报（自然科学版）》2022年第2期82-89,共8页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(32070359);中国科学院大学生创新实践训练计划项目(KCJH-80107-2020-004 No.97);国家留学基金项目(202004910143);广东省自然科学基金面上项目(2021A1515012410)。

摘　　要：单染色体识别为植物遗传学、生物多样性和进化研究的重要方向,也是大型基因组多倍体植物研究的难题之一。文章综述了单拷贝序列染色体涂染(single-copy sequence based chromosome painting)和寡核苷酸荧光原位杂交(oligonucleotide-FISH,Oligo-FISH)技术合成新型探针池的方法,总结其在比较基因组学研究中的应用案例,提出开发近缘物种通用寡核苷酸探针库的研究趋势,并对植物染色体分子核型技术在植物系统发育重建和植物育种中的应用进行了展望。In recent years,the individual chromosome identification has become an important direction in plant genetics,biodiversity,and evolution studies,and it is also one of difficult problems in the study of large-genome polyploidy plants with chromosomes of similar morphology.Here,the design and application of novel synthetic probe pools in comparative genomics research were reviewed,based on single-copy sequence-based chromosome painting and oligonucleotide fluorescent in situ hybridization(Oligo-FISH).The exploration of new approaches promoting plant diversification studies through chromosomal molecular karyotype research was discussed.The applications of plant chromosome molecular karyotype technology in plant phylogenetic reconstruction and plant breeding were proposed.

关 键 词：染色体分子核型 染色体间重组 荧光原位杂交 单拷贝寡核苷酸序列 重复序列 

分 类 号：Q943[生物学—植物学]