基于两种棘球蚴绦虫感染的小鼠肝组织病灶早期钙化比较  

Comparison of calcification in liver tissue lesions of mice infected with two kinds of Echinococcus at different time

作　　者：高风亦 邢稚坤 谭小武 俞晓凡 杨海成 赵雪源 廖原 陈雪玲 吴向未[1] GAO Fengyi;XING Zhikun;TAN Xiaowu;YU Xiaofan;YANG Haicheng;ZHAO Xueyuan;LIAO Yuan;CHEN Xueling;WU Xiangwei(Department of General Surgery,the First Affiliated Hospital,School of Medicine,Shihezi University,Shihezi,Xinjiang 832008,China;Department of Immunology,School of Medicine,Shihezi University,Shihezi,Xinjiang 832008,China)

机构地区：[1]石河子大学医学院第一附属医院肝胆外科,新疆石河子832008 [2]石河子大学医学院免疫学教研室,新疆石河子832008

出　　处：《石河子大学学报（自然科学版）》2021年第6期748-754,共7页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(81760570,81760371)。

摘　　要：目的通过对泡状棘球蚴原头节、细粒棘球蚴原头节对小鼠肝脏的感染进行模拟并按不同时间节点观察感染灶,比较分析两种棘球蚴原头节感染小鼠肝组织病灶模型的钙化方式、钙化程度的差异,进一步验证并明确两种棘球蚴感染小鼠肝组织的病理转归的差异。方法将泡球蚴原头节和细粒棘球蚴原头节通过肝被膜下注射打入C57BL/6小鼠肝脏内,分别按30、90、180 d 3个时间节点针对被感染小鼠的肝组织制作组织切片,并对组织切片进行H-E染色、茜素红染色、BMP-2免疫组化染色后,对实验结果进行半定量检测并统计分析比较两组感染肝组织钙化的差异。结果棘球蚴感染一定时间后的肝组织病灶有钙化,且钙化主要分布于病灶内囊壁;两组棘球蚴原头节感染的肝组织病灶的钙化程度均随着感染时间的延长而增加;细粒棘球蚴原头节感染病灶组的钙化程度较泡状棘球蚴原头节感染病灶组的钙化程度高。结论两种棘球蚴感染肝组织的病灶均形成了纤维钙化囊壁,钙化主要位于内囊,细粒棘球蚴感染病灶的钙化程度较泡状棘球蚴感染病灶的钙化程度更高,更易形成完整的内囊壁钙化,这间接反映了两种棘球蚴在肝组织内侵袭方式和转归的不同。Objective By simulating the infection of Echinococcus multilocularis and Echinococcus granulosus on the liver of mice,observing the infection focus at different time points,we compared and analyzed the difference of calcification mode and calcification degree between the two kinds of Echinococcus protoscolex infected mice,in order to further verify and clarify the difference of pathological changes in liver tissue of mice infected with echinococcosis.Methods The protoscolex of Echinococcus granulosus and Echinococcus multilocularis were injected into the liver of C57BL/6 mice under the liver capsule.The liver tissue sections of the infected mice were made at 30 days,90 days and 180 days respectively.After H-E staining,alizarin red staining and BMP-2 immunohistochemical staining,the experimental results were semi quantitatively detected and statistically analyzed the difference of calcification in infected liver tissue between the two groups.Results Calcification was found in the liver tissue after echinococcal infection for a certain period of time,and the calcification was mainly distributed in the inner wall of the lesion;The degree of calcification of hepatic lesions in the two groups increased with the extension of infection time;The degree of calcification in Echinococcus granulosus group was higher than that in Echinococcus alveolar group.Conclusions The lesions infected with Echinococcus granulosus had a higher degree of calcification than those infected with Echinococcus granulosus,which indirectly reflected the different invasion and outcome of the two Echinococcus species in liver tissue.

关 键 词：泡状棘球蚴 细粒棘球蚴 钙化 骨形态发生蛋白-2 

分 类 号：R602[医药卫生—外科学] R372[医药卫生—临床医学]