甜玉米籽粒总RNA提取方法的比较  

Comparison of RNA Extraction Methods from Sweet Corn Kernels

作　　者：于惠琳[1] 吴玉群[1] 王延波[1] 张洋[1] YU Hui-lin(Maize Research Institute,Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161)

机构地区：[1]辽宁省农业科学院玉米研究所,辽宁沈阳110161

出　　处：《园艺与种苗》2022年第1期67-68,71,共3页Horticulture & Seed

基　　金：辽宁省自然科学基金项目(2019-MS-192);国家重点研发计划(2016YFD0101203-1);院长基金项目(2021HQ1905)。

摘　　要：[目的]探索获得高质量、高纯度的甜玉米籽粒总RNA的提取方法。[方法]以授粉后20 d的超甜玉米系SY01籽粒为试验材料,比较了RNAiso法、Trizol法、CTAB法3种方法对甜玉米籽粒总RNA的提取效果,用琼脂糖凝聚电泳、Nanodrop2000、Agilent 2100 Bioanalyzer检测了提取的RNA的完整性、纯度和浓度。[结果]RNAiso法提取的总RNA样品,OD_(260/280)值为2.09,浓度为1 250 ng/μL,RIN值为8.6,条带清晰完整,获得率高,质量好。[结论]RNAiso法适合玉米籽粒总RNA的大量提取,总RNA产物可直接用于Micro RNA和转录组测序等试验。[Objective] To explore the extraction method of total RNA from sweet corn kernels with high quality and purity. [Method] The total RNA of sweet corn kernels was extracted by RNAiso method using super sweet corn line SY01 kernels at 20 d after pollination as the test material,we compared the effects of three methods,RNAiso method,Trizol method and CTAB method,on the extraction of total RNA from sweet corn kernels. The integrity,purity and concentration of the extracted RNA were examined using agarose coagulation electrophoresis,Nanodrop 2000 and Agilent 2100 Bioanalyzer. [Result] The total RNA samples extracted by RNAiso method had an OD_(260/280) value of 2.09,a concentration of 1 250 ng/μL,a RIN value of8.6,clear and complete bands,high yield and good quality. [Conclusion] RNAiso method is suitable for bulk extraction of total RNA from corn kernel,and the total RNA products can be directly used for experiments such as microRNA and transcriptome sequencing.

关 键 词：甜玉米 籽粒 总RNA 

分 类 号：S513[农业科学—作物学]