重离子诱变微生物育种的研究进展  

Advanced in Microbial Breeding by Heavy Ion Mutation

作　　者：汶瑛 于雪 吴玉洁 张威 陈拓 刘光琇 WEN Ying;YU Xue;WU Yu-jie;ZHANG Wei;CHEN Tuo;LIU Guang-xiu(Key Lab.of Desert&Desertif.,NW Inst.of Eco-Environ.&Res.,Chinese Acad.of Sci.,Lanzhou 730000;Uni.of Chinese Acad.of Sci.,Beijing 100049;Key Lab.of Extr.Environ.Microbial Res.&Engin.,NW Inst.of Eco-Environ.&Res.,Chinese Acad.of Sci.,Lanzhou 730000)

机构地区：[1]中国科学院西北生态环境资源研究院沙漠与沙漠化重点实验室,甘肃兰州730000 [2]中国科学院大学,北京100049 [3]中国科学院西北生态环境资源研究院甘肃省极端环境微生物资源与工程重点实验室,甘肃兰州730000

出　　处：《微生物学杂志》2021年第6期66-74,共9页Journal of Microbiology

基　　金：国家自然科学基金项目(31870479);甘肃省科技计划项目(20JR5RA548,20YF3WA007)。

摘　　要：近年来,重离子束作为一种新的辐射诱变源在微生物育种领域已多有应用。与传统的辐照源相比,重离子束具有更高的传能线密度,可以产生更强的辐射损伤生物效应,因此诱变效率高。本文综述了近年来重离子诱变在微生物育种中取得的进展、诱变后突变体菌株的筛选策略、重离子束引起微生物遗传物质改变的直接和间接机制以及突变后的修复机理,并对其在微生物育种中存在的问题进行了探讨。Recently heavy ion beam as a novel source for radiation mutation was widely applied in biological mutagenesis breeding.As compared with the traditional irradiation source,heavy ion beam possess higher linear energy transferring density that could produce stronger radio-bio-injury effect,therefore,it has high mutation efficiency.This article reviewed recent advanced in heavy ion mutagenesis in microbial breeding,the screening strategies of mutant strains after mutation,direct and indirect mechanism of the change of microbial genetic material caused by heavy ion beam,and discussed the existing problems in microbial breeding.

关 键 词：重离子 微生物育种 筛选策略 DNA双链断裂 诱变机制 

分 类 号：Q939.96[生物学—微生物学]