藻栖桔绿木霉中一个三醇类新化合物的分离鉴定与生物活性研究  

Isolation,Identification,and Bioactivity of A New Triol from Algicolous Fungus Trichoderma citrinoviride

作　　者：刘向红[1,2] 季乃云 LIU Xianghong;JI Naiyun(Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院烟台海岸带研究所,山东烟台264003 [2]中国科学院大学,北京100049

出　　处：《化学与生物工程》2022年第2期15-18,共4页Chemistry & Bioengineering

基　　金：国家自然科学基金面上项目(42076096)。

摘　　要：从山东威海海域的松节藻科海洋红藻冈村凹顶藻中分离得到一株内生真菌桔绿木霉A-WH-20-3。在批量发酵的基础上,用有机溶剂提取获得粗提物;利用硅胶柱层析、RP-18反相硅胶柱层析、Sephadex LH-20凝胶柱层析、制备薄层层析、高效液相色谱等手段,从粗提物中分离获得一个新的三醇类化合物;利用一维/二维核磁共振、高分辨质谱、红外光谱等谱学技术对其结构进行鉴定,并对其生物活性进行测试。结果表明,该新化合物为(2S*,3S*,5R*,8E)-2-甲基癸-8-烯-1,3,5-三醇;其对哈维氏弧菌和灿烂弧菌具有弱抗菌活性,对海洋浮游植物赤潮异湾藻具有一定的抑制作用。We isolated an endophytic fungus Trichoderma citrinoviride A-WH-20-3 from the marine red alga Laurencia okamurai(Rhodomelaceae)collected from the coast of Weihai in Shandong Province.On the basis of batch fermentation,we extracted the crude extracts with organic solvents.Moreover,we isolated a new triol from crude extracts by silica gel column chromatography,RP-18 reverse phase silica gel column chromatography,Sephadex LH-20 gel column chromatography,preparative thin layer chromatography,and high performance liquid chromatography.Furthermore,we identified its structure by 1D/2D nuclear magnetic resonance,high-resolution mass spectrometry,and infrared spectroscopy,and tested its bioactivity.The results show that the new triol is identified as(2S*,3S*,5R*,8E)-2-methyl-8-decene-1,3,5-triol,which has weak antibacterial activity against V.harveyi and V.splendidus,and has a certain inhibitory effect on marine phytoplankton Heterosigma akashiwo.

关 键 词：木霉 藻栖真菌 桔绿木霉 结构鉴定 生物活性 

分 类 号：TQ465.92[化学工程—制药化工] O629[理学—有机化学]