NAA处理对艾纳香扦插生根生理生化特性的影响  

Effects of NAA on Physiological and Biochemical Characteristic of Blumea balsamifera during Cutting Rooting

作　　者：黄梅[1] 陈振夏[1] 于福来[1] 王凯[1] 肖永锋 庞玉新 陈鸿发 HUANG Mei;CHEN Zhenxia;YU Fulai;WANG Kai;XIAO Yongfeng;PANG Yuxin;CHEN Hongfa(Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences/Hainan Provincial Engineering Research Center for Blumea balsamifera,Haikou 571101,China;School of Chinese Material Medical Resources,Guangdong Pharmaceutical University,Yunfu527500,Guangdong,China;Hainan Ainaxiang Biotechnology Development Co.LTD,Haikou 570208,China)

机构地区：[1]中国热带农业科学院热带作物品种资源研究所/海南省艾纳香工程技术研究中心,海口571101 [2]广东药科大学中药资源学院,广东云浮527500 [3]海南艾纳香生物科技发展股份有限公司,海口570208

出　　处：《热带亚热带植物学报》2022年第1期97-103,共7页Journal of Tropical and Subtropical Botany

基　　金：海南省自然科学基金项目(319QN312,2019CXTD414);农业农村部品种改良提升专项子任务(RZJP202006);国家热带植物种质资源库项目资助。

摘　　要：为探讨NAA对艾纳香(Blumea balsamifera)扦插生根的影响,4 a生艾纳香健康枝条用500 mg/L NAA处理,对生根过程中的生理生化特征进行了研究。结果表明,艾纳香扦插生根率与内源IAA、GA含量和IAA/ABA呈正相关,而与ABA含量呈负相关。NAA处理能提高插穗的IAA含量,降低ABA含量,有助于不定根的产生。插穗生根率与多酚氧化酶(PPO)活性正相关,与吲哚乙酸氧化酶(IAAO)活性负相关。NAA处理能够提高插穗PPO活性,降低IAAO活性,对插穗生根有利。可溶性蛋白、可溶性总糖含量与生根率均呈正相关关系。NAA处理可促进插穗的可溶性总糖、可溶性蛋白的积累,提高插穗的细胞分化和代谢能力。因此,500 mg/L NAA处理插穗可促进不定根的生长发育。In order to explore the effect of NAA on cutting rooting of Blumea balsamifera, the changes in physiological and biochemical characteristics of 4-year-old healthy branches as cuttings were studied treated with 500 mg/L NAA. The results showed that rooting rate was positively correlated with endogenous IAA, GA content and IAA/ABA, but negatively with ABA content. The IAA content of cuttings increased and ABA content decreased treated with NAA, showing that NAA had helped with adventitious roots. The rooting rate of cuttings was positively correlated with PPO activity and negatively with IAAO activity, and NAA could enhance PPO activity and reduce IAAO activity of cuttings, which is beneficial to rooting. The contents of soluble protein and total soluble sugar were positively correlated with rooting rate. NAA could promote the accumulation of total soluble sugar and soluble protein, improve the cell differentiation and metabolic ability of cuttings. Therefore,500 mg/L NAA could promote the growth and development of adventitious roots.

关 键 词：艾纳香 扦插 NAA PPO IAAO 营养物质 

分 类 号：S567.239[农业科学—中草药栽培]