三叶木通藤茎的三萜成分及其抗菌活性研究  

Triterpenes from Stems of Akebia trifoliata and Their Antibacterial Activities

作　　者：贾永霞[1] 杨秋霞 曾雷[2] 徐巧林[2] 刘少博 徐信兰[1] 谭建文 JIA Yongxia;YANG Qiuxia;ZENG Lei;XU Qiaolin;LIU Shaobo;XU Xinlan;TAN Jianwen(South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China;Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangdong Academy of Forestry,Guangzhou 510520,China;College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]中国科学院华南植物园,广州510650 [2]广东省林业科学研究院广东省森林培育与保护利用重点实验室,广州510520 [3]华南农业大学林学与风景园林学院,广州510642

出　　处：《热带亚热带植物学报》2022年第1期144-150,共7页Journal of Tropical and Subtropical Botany

基　　金：国家自然科学基金项目(31470422,31500291);广东省林业科技创新项目(2020KJCX013);广东省自然科学基金项目(2019A1515011236)资助。

摘　　要：为阐明三叶木通(Akebia trifoliata)藤茎的化学成分及其抗菌物质基础,采用正、反相硅胶柱色谱、凝胶柱色谱及半制备液相色谱等方法对其化学成分进行研究,从其乙醇提取物中得到9个三萜类化合物,根据理化性质和波谱数据,其结构分别鉴定为stachlic acid A(1),齐墩果酸(2)、乌苏酸(3)、2α,3β-dihydroxyolean-13(18)-en-28-oic acid(4)、serratagenic acid(5)、gypsogenic acid(6)、20α-hydroxyl-29-noroleanolic acid(7)、mesembryanthemoidigenic acid(8)和12α-hydroxy-δ-lactone(9)。抗菌活性分析表明,化合物2和3对革兰氏阳性菌金黄色葡萄球菌、蜡样芽孢杆菌和枯草芽胞杆菌有显著的抗菌活性,但对革兰氏阴性菌鼠伤寒沙门氏菌无明显抑制作用。化合物3和7为首次从木通属中分离得到,化合物1、4、6和9为首次从该植物中分离获得。To study triterpenes from Akebia trifoliata and its antibacterial activities, nine triterpenes were isolated from the ethanol extract by various methods, such as silica gel, ODS, Sephadex LH-20 column chromatography and semi-preparative HPLC. Based on physical and chemical properties and spectral data, their structures were identified as stachlic acid A(1), oleanolic acid(2), ursolic acid(3), 2α,3β-dihydroxyolean-13(18)-en-28-oic acid(4), serratagenic acid(5), gypsogenic acid(6), 20α-hydroxyl-29-noroleanolic acid(7), mesembryanthemoidigenic acid(8) and 12α-hydroxy-δ-lactone(9). The compounds 2 and 3 had strong antibacterial activity against three tested bacterial strains in vitro. Compounds 1, 4, 6 and 9 were isolated from A. trifoliata for the first time,compounds 3 and 7 were isolated from genus Akebia for the first time.

关 键 词：三叶木通 藤茎 三萜 抗菌活性 

分 类 号：R284.1[医药卫生—中药学]