内碳源短程反硝化启动及EPD-ANAMMOX耦合工艺性能  

Start-up of endogenous partial denitrification and performance of EPD-ANAMMOX coupling process

作　　者：张小玲[1,2] 张萌[1] 陈紫薇 张卓[1] ZHANG Xiao-ling;ZHANG Meng;CHEN Zi-wei;ZHANG Zhuo(School of Water and Environment,Chang’an University,Xi’an 710064,China;Key Laboratory of Subsurface Hydrology and Ecological Effects in Arid Region of the Ministry of Education,Chang’an University,Xi’an 710064,China)

机构地区：[1]长安大学水利与环境学院,陕西西安710064 [2]长安大学,旱区地下水与生态效应教育部重点实验室,陕西西安710064

出　　处：《中国环境科学》2022年第2期601-611,共11页China Environmental Science

基　　金：国家自然科学基金资助项目(51408041)。

摘　　要：接种普通活性污泥,以乙酸盐为碳源,控制进水COD/P为150:1,在A/O SBR反应器内富集培养了聚糖菌;采用逐渐提高SBR厌氧末硝酸盐投加浓度的方法,将聚糖菌驯化诱导为反硝化聚糖菌结果.SBR厌氧末排水中COD与缺氧末排水基本相同,COD平均去除率达到86.74%,总氮去除率达到98%以上.然后缩短SBR的厌氧及缺氧时间,即可启动内碳源短程反硝化(EPD)系统,缺氧末亚硝酸盐转化率(NTR)平均值为65.96%;表明内碳源短程反硝化与厌氧氨氧化(EPD-ANAMMOX)耦合工艺运行的30d内,COD去除负荷及去除率分别为0.337kgCOD/(kgMLSS·d)及87.21%;总氮去除负荷及去除率分别为0.222kgN/(m^(3)·d)及86.12%,出水中NO_(3)^(-)-N浓度低至4.2mg/L,NO_(2)^(-)-N和NH_(4)^(+)-N浓度接近于零.通过高通量测序,EPD-SBR稳定运行期的污泥与接种污泥相比,具有反硝化功能的聚糖菌属Competibacter丰度从0.001%增加至25.06%,其它聚糖菌属Defluviicoccus、Contendobacter、Sphingobium以及Amaricoccus的总丰度从0.14%增加至0.431%,表明Competibacter是SBR反应器内内碳源短程反硝过程的主要功能菌属.This study was designed to evaluate performances of the start-up of endogenous partial denitrification and EPD-ANAMMOX process.The glycogen accumulation bacteria(GAO)were enriched in the anaerobic/aerobic sequence batch reactor(SBR)using the activated sludge as inoculum sludge and the acetate acted as carbon source by regulating the influent COD/P ratio as 150:1.Then,the GAOs were induced into denitrifying glycogen accumulation bacteria(DGAO)by means of gradually boosting the concentration of added nitrate at the end of anaerobic phase in SBR.Correspondingly,the level of chemical oxygen demand(COD)at the end of anoxic period was almost the same as that at the distal end of anaerobic stage,the total nitrogen removal efficiency and the average COD removal efficiency were over 98%and 86.74%at the end of anaerobic period of SBR,respectively.Furthermore,the endogenous partial denitrification system was successfully formed by shortening the anaerobic and anoxic time,the ratio of nitrite translated from nitrate(NTR)at the end of anoxic period was up to 65.96%.The average total nitrogen and COD removal load in the 30days operation of the coupled process were 0.222kgN/(m^(3)⋅d)and 0.337kgCOD/(kgMLVSS.d)with an efficiency of 86.12%and 87.21%,respectively.Typically,the effluent NO_(3)^(-)-N concentration was lower than 4.2mg/L and both NO_(2)^(-)-N and NH_(4)^(+)-N levels were near to 0mg/L in the same phase.Compared with inoculated sludge,the relative abundance of competibacter increased from 0.001%to 25.06%in the sludge of EPD-SBR during the stable operation period,and the total abundance of Defluviicoccus,Contendobacter,Sphingobium,and Amaricoccus increased from 0.14%to 0.431%,implying that the competibacter was the dominated functional bacteria for the endogenous partial denitrification system.

关 键 词：内碳源短程反硝化 亚硝酸盐转化率 反硝化聚糖菌 厌氧氨氧化 

分 类 号：X703.5[环境科学与工程—环境工程]