反硝化型甲烷厌氧氧化(DAMO)系统pH值耦合模型研究  

pH coupling model of denitrifying anaerobic methane oxidation(DAMO)system

作　　者：吕娇 楼菊青[1] 徐帆 LU Jiao;LOU Ju-qing;XU Fan(College of Environmental Science and Engineering,Zhejiang Gongshang University,Hangzhou 310018,China)

机构地区：[1]浙江工商大学环境科学与工程学院,浙江杭州310018

出　　处：《中国环境科学》2022年第2期612-619,共8页China Environmental Science

基　　金：浙江省自然科学基金资助项目(LY21D030003);浙江工商大学研究生科研创新基金一般项目(19020160029)。

摘　　要：对3个具有不同优势菌种反应器中厌氧氧化(DAMO)过程与pH值进行动力学耦合,结果表明,在25℃,Anammox-DAMO混培系统最大脱氮速率、硝酸盐初始抑制浓度和铵盐初始抑制浓度分别为3.95mg/(L·d),182.63,196.40mg/L;Nitrate-DAMO系统最大脱氮速率、硝酸盐初始抑制浓度分别为4.30mg/(L·d),367.69mg/L;Nitrite-DAMO系统最大脱氮速率、亚硝酸盐初始抑制浓度分别为4.04mg/(L·d),293.35mg/L.脱氮速率均随pH值增加先增大后减小,3个系统最佳脱氮pH值分别为7.5±0.2,7.2±0.2,7.8±0.2.脱氮动力学表明,3个不同系统DAMO反应过程均可用Haldane-pH值耦合模型描述.Nitrate-DAMO系统、Nitrite-DAMO系统脱氮过程还可用Monod-pH值耦合方程描述,Nitrate-DAMO系统细菌生长速率、硝酸盐亲和常数和抑制常数分别为1291.21cfu/(L·d),295.23,72.63mg/L;Nitrite-DAMO系统细菌生长速率、亚硝酸盐亲和常数和抑制常数分别为4040.42cfu/(L·d),264.51,5.02mg/L.This study tried to couple the pH value with the reaction kinetics of the denitrification-type methane anaerobic oxidation process.The Denitrifying Anaerobic Methane Oxidation(DAMO)reaction rate and pH value were coupled and evaluated in three reactors with different dominant bacteria.The results show that the Anammox-DAMO maximum denitrification rate and the initial inhibition concentration of nitrate ammonium at 25℃were 3.95mg/(L⋅d),182.63mg/L and 196.40mg/L,respectively.The maximum denitrification rate and the initial inhibition concentration of nitrate in Nitrate-DAMO system were 4.30mg/(L⋅d)and 367.69mg/L,respectively.The maximum denitrification rate and the initial inhibition concentration of nitrite in Nitrite-DAMO system were 4.04mg/(L⋅d)and 293.35mg/L,respectively.The denitrification rate of the systems increased first and then decreased with the increase of pH.The optimum pH was 7.5±0.2,7.2±0.2,7.8±0.2,respectively.The bacterial growth rate,nitrate affinity constant and inhibition constant of Nitrate-DAMO system were 1291.21cfu/(L⋅d),295.23mg/L and 72.63mg/L,respectively;The bacterial growth rate,nitrite affinity constant and inhibition constant of Nitrite-DAMO system were 4040.42cfu/(L⋅d),264.51mg/L and 5.02mg/L,respectively.All the three DAMO systems could be described by the coupled Haldane pH model;the denitrification process of Nitrate-DAMO system and Nitrite-DAMO system could also be described by Monod-pH coupling equation.

关 键 词：反硝化型甲烷厌氧氧化 脱氮性能 Hanldane方程 MONOD方程 PH值 耦合模型 

分 类 号：X703[环境科学与工程—环境工程]