多环芳烃共代谢对苯并[a]蒽微生物降解的影响及机制  

Effect of co-metabolism by polycyclic aromatic hydrocarbon on the microbial degradation of benzo[a]anthracene and its mechanism

作　　者：朱清禾[1,2] 曾军 吴宇澄[1] 杨洁[2] 林先贵 ZHU Qing-he;ZENG Jun;WU Yu-cheng;YANG-Jie;LIN Xian-gui(Key Laboratory of Soil Environmental and Pollution Remediation,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,China;State Environmental Protection Engineering Center for Urban Soil Contamination Control and Remediation,Shanghai Academy of Environmental Sciences,Shanghai 200233,China)

机构地区：[1]中国科学院南京土壤研究所,土壤环境与污染修复重点实验室,江苏南京210008 [2]上海市环境科学研究院,国家环境保护城市土壤污染控制与修复工程技术中心,上海200233

出　　处：《中国环境科学》2022年第2期808-814,共7页China Environmental Science

基　　金：国家重点研发计划(2019YFC1803700);国家自然科学基金资助项目(41977132);上海市科技人才计划项目(19XD1434900)。

摘　　要：利用^(14)C同位素示踪技术,研究土壤中菲、蒽、芘3种底物对苯并[a]蒽(BaA)环境归趋的影响,高通量测序解析细菌群落响应.结果表明,3种底物均可以促进BaA的降解,添加蒽作为共代谢底物后,BaA矿化率比对照提高了2.5%,而添加菲、芘时,^(14)C在土壤的结合态比对照升高4%,达总添加量的31%.共代谢底物改变了细菌共生网络的拓扑结构:添加底物蒽后,网络中的正连接比例显著升高,物种间以共生关系为主;菲、芘作为共代谢底物时,细菌物种间的负连接比例升高,暗示微生物种群竞争变激烈.推测蒽与BaA化合物结构相似并有相近的代谢途径,细菌共生性增强并直接促进了BaA的矿化.In this study,the effect of three co-metabolic substrates(phenanthrene,anthracene,pyrene)on environmental distribution of benzo[a]anthracene(BaA)was studied by using ^(14)C isotope tracer.High-throughput sequencing and co-occurrence network analysis were used to evaluate bacterial community responses.The results demonstrated that all the three co-metabolic substrates promoted degradation of BaA.The mineralization of BaA increased by 2.5%in the anthracene treatment compared to the control.In the treatments of phenanthrene and pyrene,the bound residue of ^(14)C was 4%higher than in the control,amounting to 31%of the total addition.The topological characteristics of bacterial co-occurrence network changed with the supplementation of co-metabolic substrates.Anthracene significantly raised the proportion of positive links,which indicated mutualistic interactions.In contrast,the competition between bacterial taxa was strengthened in the treatments of phenanthrene and pyrene,as evidenced by increased negative links after incubation.It was likely that anthracene and BaA degraded through common metabolic pathways,which caused mutualistic interactions and higher mineralization rate of BaA in the soil microcosms.Overall,co-metabolism could be an important factor influencing the fate of BaA in soil and changing the interaction among bacterial species.

关 键 词：苯并[a]蒽 环境归趋 共代谢 细菌共生网络 

分 类 号：X53[环境科学与工程—环境工程]