外源甲基乙二醛对干旱胁迫下板栗幼苗的影响  

Effects of exogenous methylglyoxal on chesnut seedlings under drought stress

作　　者：孙晓莉[1] 贾春燕 田寿乐[1] 徐文燕 王金平 冉昆[1] 沈广宁[1] SUN Xiao-li;JIA Chun-yan;TIAN Shou-le;XU Wen-yan;WANG Jin-ping;RAN Kun;SHEN Guang-ning(Shandong Institute of Pomology,Tai’an 271018,Shandong,China;Taishan Forest Pest Control and Quarantine Station,Tai’an 271018,Shandong,China;Daiyue District Ecological Forestry Development Center,Tai’an 271018,Shandong,China)

机构地区：[1]山东省果树研究所,山东泰安271018 [2]泰山森林病虫害防治检疫站,山东泰安271018 [3]岱岳区生态林业发展中心,山东泰安271018

出　　处：《应用生态学报》2022年第1期104-110,共7页Chinese Journal of Applied Ecology

基　　金：山东省自然科学基金项目(ZR2020MC141);山东省农业科学院农业科技创新工程项目(CXGC2021A31)资助。

摘　　要：甲基乙二醛(MG)是一种在植物中具有多种功能的新型信号分子。为探究MG对板栗幼苗干旱胁迫的影响,以两年生‘黄棚’板栗幼苗为试材,通过聚乙二醇(PEG)模拟干旱胁迫并进行MG及其清除剂N-乙酰半胱氨酸(NAC)处理,分析板栗幼苗叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)等抗氧化酶活性和乙二醛酶Ⅰ(GlyⅠ)、乙二醛酶Ⅱ(GlyⅡ)等乙二醛酶活性,检测内源MG含量、丙二醛(MDA)、过氧化氢(H_(2)O_(2))、超氧阴离子(O_(2)^(-·))等抗氧化物质含量及脯氨酸(Pro)、可溶性糖(SS)、甜菜碱(GB)等渗透调节物质含量。结果表明:外源0.5 mmol·L^(-1)MG处理可显著提高干旱胁迫下板栗幼苗叶片SOD、POD、CAT、APX、GR等抗氧化酶活性和乙二醛酶(GLyⅠ、GLyⅡ)活性,并增加叶片中Pro、SS、GB等渗透调节物质和还原型抗坏血酸(AsA)、还原型谷胱甘肽(GSH)等抗氧化物质含量,降低了MG、MDA、H_(2)O_(2)、O_(2)^(-·)、脱氢抗坏血酸(DHA)含量,从而减少了干旱胁迫对植株造成的膜脂过氧化损伤,缓解渗透胁迫,提高了板栗对干旱的适应性,而添加MG清除剂NAC抑制了这种效果。表明MG对改善植物的抗旱性具有积极作用。本研究结果可为进一步探索MG缓解板栗干旱胁迫的机理提供理论依据。Methylglyoxal(MG)is a novel signaling molecule with multiple functions in plants.To explore the effects of MG on Chinese chestnut(Castanea mollissima)under drought stress,two-year-old‘Huangpeng’chestnut seedlings were treated with 15%polyethylene glycol(PEG)coupled with MG or its scavenger N-acetyl-L-cys-teine(NAC).We measured the activities of antioxidant enzymes,including superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),ascorbate peroxidase(APX)and glutathione reductase(GR),and glyoxalase enzymes,including glyoxalaseⅠ(GlyⅠ)and glyoxalaseⅡ(GlyⅡ).Contents of antioxidants such as endogenous MG,malondialdehyde(MDA),H_(2)O_(2),and O_(2)^(-·)as well as the osmotic adjustment substances including proline(Pro),soluble sugar(SS),glycine betaine(GB)were also detected.The results showed that 0.5 mmol·L^(-1)MG significantly increased the activities of antioxidant enzymes(SOD,POD,CAT,APX,GR)and glyoxalase enzymes(GlyⅠ,GlyⅡ)in leaves of chestnut seedlings under drought stress,elevated the contents of osmotic adjustment substances(Pro,SS,GB)and antioxidant substances(ASA,GSH),and reduced the contents of MG,MDA,H_(2)O_(2),O_(2)^(-·)and dehydroascorbate(DHA).Drought stress induced damages such as membrane lipid peroxidation and osmotic stress was alleviated by MG,leading to an overall improved adaptability of chestnut to drought stress.Moreover,the addition of MG scavenger NAC could reverse the effects induced by MG,indicating that MG had positive impacts on drought resistance of chestnut plants.Our study provided a theoretical basis for further exploring the mechanism of MG in alleviating drought stress induced symptoms in chestnut.

关 键 词：甲基乙二醛 干旱胁迫 抗氧化 乙二醛酶 渗透调节 

分 类 号：S664.2[农业科学—果树学]