AM真菌对菌根化牡丹组培苗矿质元素吸收的影响  

Effects of AM Fungi on Mineral Element Uptake in Tissue Culture Seedlings of Paeonia suffruticosa

作　　者：曾端香 王莲英[2] ZENG Duanxiang;WANG Lianying(National Academy of Forestry and Grassland Administration,Beijing 102600;College of Landscape Architecture,Beijing Forestry University,Beijing 100083)

机构地区：[1]国家林业和草原局管理干部学院,北京102600 [2]北京林业大学园林学院,北京100083

出　　处：《中国农学通报》2022年第1期53-58,共6页Chinese Agricultural Science Bulletin

基　　金：国家林业局重点研究项目“牡丹新优品种繁育及区域化试验”;国家林业和草原局“百千万人才工程”省部级人选(2020)。

摘　　要：为探索AM真菌在牡丹组培上的应用效益及机理,保存和扩繁新优杂交牡丹资源及提供解决牡丹组培中生根难、移栽成活率低等瓶颈问题的新途径,选用牡丹新优杂交系种子进行胚培养得到胚根苗无菌体系,在瓶内对胚根苗接种AM真菌获得菌根化组培苗,继而研究AM真菌对牡丹组培苗生长发育和矿质元素吸收的影响。结果表明,AM真菌能显著促进牡丹组培苗菌根化和矿质元素的吸收,Gg+Gm的混合孢子接种的侵染率(93.12%)、侵染强度(31.23)极显著(P=0.01)高于Gm、Gg和CK;AM真菌显著促进了牡丹‘Z1-10-3’组培苗的N、P、K、Fe、Ca的吸收,但却降低了Mg、Mn的含量。接种Gg+Gm改善P的吸收效果最显著,比CK增长了63.4%,N、K、Fe含量分别增长了47.2%、47.3%、31.2%。接种Gm、Gg和Gg+Gm对矿质元素含量的影响趋势大体一致,但以混合接种剂Gg+Gm增效作用最显著。研究结果可为牡丹组培快繁工厂化育苗提供有效途径和依据。The study aims to explore the inoculation effect and mechanism of arbuscular mycorrhizal fungi(AMF)on tissue culture seedlings of Paeonia suffruticosa,conserve and multiply germplasm resources of hybrid strain of P.suffruticosa,and provide means to solve bottleneck problems such as difficult rooting of in vitro plants and low transplantation survival rate,etc.We took embryo-culture and got the sterile systems with seeds of the new optimal hybrid strain of P.suffruticosa,then obtained mycorrhizal tissue culture seedlings by inoculating different AMF plantlets in vitro,and studied the effects of AM fungi on the growth and absorption of mineral elements in mycorrhizal seedlings.The results showed that,the mixture spore of Gg+Gm had the highest colonization frequency(93.12%)and colonization intensity(31.23),which were significantly higher than those of Gg,Gm and CK(P=0.01).AMF could improve the mycorrhization and absorption of mineral elements in P.suffruticosa tissue culture seedlings,and significantly promote the absorption of N,P,K,Fe and Ca of tissue culture seedlings of‘Z1-10-3’,but decrease the content of Mg and Mn.Gg+Gm had the most significant improvement effect on absorption of P,which was increased by 63.4%compared with that of CK,and N,K and Fe contents were increased by 47.2%,47.3%and 31.2%,respectively.The effects of inoculation of Gm and Gg consisted with that of Gg+Gm with a smaller change range.However,Gg+Gm had the most significant synergistic effect.The study can provide an effective way for rapid propagation of seedlings of P.suffruticosa.

关 键 词：牡丹 AM真菌 菌根化组培苗 丛枝菌根侵染 矿质元素 

分 类 号：S685.11[农业科学—观赏园艺]