间质性膀胱炎/膀胱疼痛综合征膀胱组织中miR-214的表达及意义  

Expression and significance of miR-214 in the bladder tissues of interstitial cystitis/bladder pain syndrome

作　　者：王春辉 李春生 高志明 郭俊生 张俊毅 蒋莹 包国昌 WANG Chunhui;LI Chunsheng;GAO Zhiming;GUO Junsheng;ZHANG Junyi;JIANG Ying;BAO Guochang(Department of Urology,Affiliated Hospital of Chifeng University,Urology Research Center,Chifeng,Inner Mongolia,024000,China;Department of Pathology,Affiliated Hospital of Chifeng University;Department of Reproduction,Affiliated Hospital of Chifeng University)

机构地区：[1]赤峰学院附属医院泌尿外科,赤峰学院泌尿外科研究所,内蒙古赤峰024000 [2]赤峰学院附属医院病理科 [3]赤峰学院附属医院生殖医学科

出　　处：《临床泌尿外科杂志》2021年第12期961-964,共4页Journal of Clinical Urology

基　　金：内蒙古自治区高等学校科学技术研究项目(No:NJZY20197)。

摘　　要：目的:探讨miR-214在间质性膀胱炎/膀胱疼痛综合征(IC/BPS)患者膀胱组织中的表达及意义。方法:30例IC/BPS患者,根据膀胱镜下表现,分为Hunner溃疡组(n=12)和点状出血组(n=18)。分别取病变组织和病变周围组织行实时定量逆转录聚合酶链反应(qRT-PCR)。分别比较两组中病变组织与其周围组织中miR-214的表达情况,以及两组间病变组织中miR-214的表达情况。结果:Hunner溃疡病变处组织miR-214表达水平0.49~1.23,平均(0.89±0.20),Hunner溃疡病变周围组织miR-214表达水平2.78~4.81,平均(3.78±0.63)。点状出血处病变组织miR-214表达水平0.94~1.84,平均(1.47±1.33),点状出血病变周围组织miR-214表达水平2.76~3.95,平均(3.60±0.42)。Hunner溃疡病变组织miR-214表达明显低于其周边组织。差异有统计学意义(t=18.76,P<0.000 1)。点状出血处病变组织miR-214表达明显低于其周边组织,差异有统计学意义(t=17.30,P<0.000 1)。Hunner溃疡病变组织miR-214表达明显低于点状出血处病变组织,差异有统计学意义(t=5.554,P<0.000 1)。结论:IC/BPS患者膀胱病变组织处较其周围组织miR-214表达明显降低,Hunner溃疡病变组织miR-214表达明显低于点状出血处病变组织。这可能与疾病的发生、发展有关。Objective: To investigate the expression and significance of miR-214 in the bladder tissues of patients with interstitial cystitis/bladder pain syndrome(IC/BPS). Methods: Thirty IC/BPS patients were divided into Hunner’s ulcer group(n=12) and punctate hemorrhage group(n=18) according to cystoscopy. Real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR) was performed on the diseased tissues and the surrounding tissues respectively. The expression of miR-214 in the diseased tissues and surrounding tissues of the two groups was compared, and the expression of miR-214 in the diseased tissues between the two groups was also compared. Results: The expression level of miR-214 in the tissue of Hunner’s ulcer was 0.89±0.20(0.49-1.23), and the expression level of miR-214 in the surrounding tissue of Hunner’s ulcer was 3.78±0.63(2.78-4.81). The expression level of miR-214 was 1.47±1.33(0.94-1.84) in the lesions with punctate hemorrhage, and 3.60±0.42(2.76-3.95) in the surrounding tissues with punctate hemorrhage. The expression of miR-214 in the Hunner’s ulcer was significantly lower than that in the surrounding tissues. The difference was statistically significant(t=18.76, P<0.000 1). The expression of miR-214 in the tissues at the punctate hemorrhage was significantly lower than that in the peripheral tissues, and the difference was statistically significant(t=17.30, P<0.000 1). The expression of miR-214 in the tissue of Hunner’s ulcer was significantly lower than that in the lesion of punctate hemorrhage, and the difference was statistically significant(t=5.554, P<0.000 1). Conclusion: The expression of miR-214 in the diseased tissues of IC/BPS patients was significantly lower than that in the surrounding tissues, and the expression of miR-214 in the diseased tissues of Hunner’s ulcer was significantly lower than that in the diseased tissues of punctate hemorrhage. This may be related to the occurrence and development of the disease.

关 键 词：间质性膀胱炎 膀胱疼痛综合征 MICRORNAS miR-214 上皮间质化 组织纤维化 

分 类 号：R694[医药卫生—泌尿科学]