八珍汤对人皮肤成纤维细胞表皮细胞生长因子、转化生长因子β1及血管内皮生长因子A表达的影响  

Effects of Bazhen Decoction on the Expression of EGF, TGF-β1 and VEGFA in Cultured Human Skin Fibroblasts

作　　者：孔令臻 邢捷[1] KONG Ling-zhen;XING Jie(Long Hua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai(200032),China)

机构地区：[1]上海中医药大学附属龙华医院中医外科,上海200032

出　　处：《中国中西医结合外科杂志》2022年第1期17-21,共5页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金项目(81804105);上海市青年医师培养资助计划(lh0113002)。

摘　　要：目的:观察不同浓度八珍汤对体外培养人皮肤成纤维细胞表皮细胞生长因子(EGF)、转化生长因子β1(TGF-β1)、血管内皮生长因子A(VEGFA)表达的影响。方法:采用CCK-8法测定不同浓度八珍汤对人皮肤成纤维细胞增殖的影响,确定药物浓度和用药时间,Western blotting法检测不同浓度八珍汤对人皮肤成纤维细胞EGF、TGF-β1、VEGFA表达的影响。结果:八珍汤能促进人皮肤成纤维细胞增殖,在浓度为1 mg/L~1 g/L时促进细胞增殖效果较明显,且药物干预24 h促细胞增殖效果明显(P<0.01)。与对照组比较,中浓度八珍汤(100 mg/L)组EGF、TGF-β1、VEGFA的表达明显增高(P<0.05);低浓度八珍汤(10 mg/L)组EGF、TGF-β1表达无明显差异(P>0.05),VEGFA的表达增高(P<0.05);高浓度八珍汤(1 g/L)组EGF、TGF-β1表达降低(P<0.05),VEGFA的表达无明显差异(P>0.05)。结论:中浓度(100 mg/L)八珍汤能加速人皮肤成纤维细胞增殖,促进其EGF、TGF-β1、VEGFA的表达,进而加速血管新生,促进创面肉芽组织生长,最终促进创面愈合。Objective Observe the effect of Bazhen Decoction of different concentrations on the expression of EGF,TGF-β1 and VEGFA in cultured human skin fibroblasts in vitro.Methods The CCK-8 method was used to determine the effect of different concentrations of Bazhen Decoction on the proliferation of human skin fibroblasts.The drug concentration and medication time were determined.Western blotting was used to detect the effects of different concentrations of Bazhen Decoction on the expression of EGF,TGF-β1 and VEGFA in human skin fibroblasts.Results Bazhen Decoction can promote the proliferation of human skin fibroblasts,and the effect of promoting cell proliferation is obvious when the concentration is 1 mg/L-1 g/L.The effect of drug intervention in promoting cell proliferation is obvious for 24 hours(P<0.01).Compared with the control group,the expressions of EGF,TGF-β1 and VEGFA in the medium concentration Bazhen decoction(100 mg/L)group were significantly increased(P<0.05).The expression of EGF and TGF-β1 in low concentration Bazhen Decoction(10 mg/L)group were not significantly increased(P>0.05).The expression of VEGFA was increased(P<0.05).The expression of EGF and TGF-β1 in the high-concentration Bazhen Decoction(1g/L)group decreased(P<0.05),and the expression of VEGFA was not significant(P>0.05).Conclusion The medium concentration Bazhen Decoction can accelerate the proliferation of human skin fibroblasts,promote the expression of EGF,TGF-β1 and VEGFA,thereby accelerating angiogenesis and promoting the growth of wound granulation tissue,and ultimately promote wound healing.

关 键 词：八珍汤 成纤维细胞 细胞增殖 生长因子 

分 类 号：R264[医药卫生—中医外科学]