桃核承气汤含药血清对脂多糖诱导的血管内皮细胞增殖及相关因子表达的影响  

Effect of Serum Containing Taohe Chengqi Decoction on LPS Induced Proliferation of Aortic Endothelial Cells and Expression of Related Factors

作　　者：郭燕玲 周鑫[1,2] 刘鹏 王军[1] GUO Yan-ling;ZHOU Xin;LIU Peng(First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,Tianjin(300381),China)

机构地区：[1]天津中医药大学第一附属医院,国家中医针灸临床医学研究中心,天津300381 [2]天津中医药大学研究生院,天津301617

出　　处：《中国中西医结合外科杂志》2022年第1期22-26,共5页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：天津市中医药重点领域科研项目(2019008)。

摘　　要：目的:探讨桃核承气汤含药血清对脂多糖(LPS)诱导的主动脉内皮细胞增殖及相关因子表达的影响。方法:将30只雄性SD大鼠随机分为2组,正常组与含药血清组各15只,含药血清组用桃核承气汤灌胃,正常组给予等剂量生理盐水灌胃,7 d后自腹主动脉取血,离心取血清,分装备用。用不同浓度梯度含药血清干预细胞,CCK-8法、流式细胞仪检测各浓度含药血清对内皮细胞增殖、凋亡的影响,确定10μmol/L为最佳干预浓度。将主动脉内皮细胞分为空白对照组(10%空白血清)、空白血清组(10%含药血清)、模型对照组(LPS+10%空白血清)、模型血清组(LPS+10%含药血清)。Western-blotting检测各组干预后的主动脉内皮细胞磷脂酰肌醇-3激酶(PI3K)、蛋白激酶B(Akt)、转化生长因子(TGF-β)、假性蛋白激酶3(TRIB3)蛋白的表达。结果:与空白血清组、空白对照组相比,模型对照组的PI3K、Akt、TGF-β表达均下降,TRIB3表达含量上升,差异有统计学意义(P<0.05);经桃核承气汤含药血清培养后,与模型对照组相比,模型血清组的PI3K、Akt、TGF-β表达含量上升,TRIB3表达下降,差异有统计学意义(P<0.05)。结论:桃核承气汤含药血清可以抑制TRIB3表达,促进PI3K、Akt和TGF-β表达,桃核承气汤含药血清能促进损伤内皮细胞新生,可能与PI3K/Akt信号通路有关。Objective To investigate the effect of serum containing Taohe Chengqi Decoction on the proliferation of aortic endothelial cells induced by lipopolysaccharide(LPS)and the expression of related factors.Methods 30 male SD rats were randomly divided into two groups:normal group and medicated serum group with 15 rats in each group.Medicated serum group was given Taohe Chengqi Decoction by gavage,and normal group was given the same dose of normal saline by gavage.After 7 days,blood was taken from abdominal aorta and serum was centrifuged for separate use.CCK-8 method and flow cytometry were used to detect the effects of different concentrations of drug containing serum on the proliferation and apoptosis of endothelial cells.The optimal intervention concentration was 10μmol/L.The cells were divided into blank control group(10%blank serum),blank serum group(10%medicated serum),model control group(LPS+10%blank serum)and model serum group(LPS+10%medicated serum).Western-blotting was used to detect the expression of phosphatidylinositol-3 kinase(PI3K),protein kinase B(Akt),transforming growth factor-β(TGF-β)and pseudo protein kinase 3(TRIB3)in aortic endothelial cells after intervention.Results Compared with blank serum group and blank control group,the expression of PI3K,Akt and TGF-βin model control group decreased(P<0.05)and the expression of TRIB3 increased(P<0.05).After cultured in Taohe Chengqi Decoction drug-containing serum,compared with model control group,the expression of PI3K,AKT,TGF-βin model serum group increased and the expression of TRIB3 decreased(P<0.05).Conclusion The serum containing Taohe Chengqi Decoction can inhibit the expression of TRIB3 and promote the expression of PI3K,Akt and TGF-β.The serum containing Taohe Chengqi Decoction can promote the regeneration of injured endothelial cells,which may be related to PI3K/Akt signaling pathway.

关 键 词：桃核承气汤 主动脉内皮细胞 PI3K/AKT信号通路 

分 类 号：R285.5[医药卫生—中药学]