苍白杆菌(Ochrobactrum sp.)FP1对芳香烃化合物降解特性及相关基因研究  

Studies on Degradation Characteristics of Aromatic Compounds and Its Related Degrading Genes of An Ochrobactrum sp.FP1

作　　者：刘宣汝 王永刚[2] 陈吉祥[1] 郭晓鹏[2] 罗旦 Liu Xuanru;Wang Yonggang;Chen Jixiang;Guo Xiaopeng;Luo Dan(College of Petrochemical Engineering,Lanzhou University of Technology, Lanzhou 730050, China;College of life science and Engineering,Lanzhou University of Technology, Lanzhou 730050, China)

机构地区：[1]兰州理工大学石油化工学院,甘肃兰州730050 [2]兰州理工大学生命科学与工程学院,甘肃兰州730050

出　　处：《环境科学与管理》2022年第2期19-22,共4页Environmental Science and Management

基　　金：国家自然科学基金(31272694)资助。

摘　　要：文章对分离的一株高效石油降解菌生理特性及芳香烃化合物降解特性、降解相关基因进行了研究。用细菌生理生化和16S rDNA序列分析初步鉴定FP1为苍白杆菌(Ochrobactrum sp.)。将菌株在含不同种类混合芳烃基础培养基中培养7 d后,用GC-MS分析发现菌株对芳烃类化合物有较好利用和降解能力,对苯、甲苯、乙苯、二甲苯、萘和联苯的降解率分别为100.00%、60.11%、66.00%、57.72%、67.94%、77.72%。芳香烃化合物降解相关基因有31个,而参与萘、甲苯、乙苯、二甲苯降解的基因占芳香烃化合物降解基因总数的38.72%。研究结果为芳烃污染土壤的微生物修复提供了基础。This paper explored the degradation characteristics and related genes of a high-efficiency petroleum degrading bacterium to aromatic hydrocarbon compounds.The strain was identified using physiological and biochemical and 16S rDNA sequence analysis.GC-MS and whole genome sequencing were used to analyze the degradation characteristics and the possible degradation genes for aromatic compounds.The results showed that the strain FP1 belonged to Ochrobactrum sp.The bacterium was cultured in a basic medium containing different types of mixed aromatic hydrocarbons for 7 days,and the degradation rates of benzene,toluene,ethylbenzene,xylene,naphthalene,and biphenyl were 100.00%,60.11%,66.00%,57.72%,67.94%,77.72%respectively.There are 31 genes related to the degradation of aromatic hydrocarbon compounds,and the genes involved in the degradation of naphthalene,toluene,ethylbenzene,and xylene account for 38.72%of the total number of aromatic hydrocarbon compound degradation genes.These results would provide references for the microbial remediation of the aromatic hydrocarbon compounds contaminatedsoils.

关 键 词：苍白杆菌 生理特征 多环芳烃 降解率 降解基因 

分 类 号：X53[环境科学与工程—环境工程]