翻地对羊肚菌栽培土壤真菌群落的影响  

Effects of Plowing Treatment on the Fungal Community Structure of Morchella Cultivated Soil at Different Depths

作　　者：王永元 徐红霞 李岩龙 曹秀文 WANG Yongyuan;XU Hongxia;LI Yanlong;CAO Xiuwen(Institute of Forestry Sciences,Bailongjiang Forestry Management Bureau of Gansu Province,Lanzhou,Gansu 730080;Gansu Bailongjiang National Forest Ecosystem Research Station,Zhouqu,Gansu 746300)

机构地区：[1]甘肃省白龙江林业管理局林业科学研究所,甘肃兰州730080 [2]甘肃省白龙江森林生态系统国家定位观测研究站,甘肃舟曲746300

出　　处：《北方园艺》2022年第2期79-86,共8页Northern Horticulture

基　　金：中央财政林业科技推广示范资助项目(2019ZYTG10);甘肃省林业科技资助项目(2017kj040)。

摘　　要：以羊肚菌栽培地内土壤为试材,采用Illumina HiSeq高通量测序技术分析土壤真菌群落结构的方法,研究了羊肚菌在进行栽培时翻地处理前后的土壤真菌群落结构的变化,以期为羊肚菌栽培时土壤真菌群落结构的变化提供参考依据。结果表明:4组土壤样品的真菌群落在门分类水平结构分析中共有优势菌门3种,分别为子囊菌门(Ascomycota)、鞭毛菌门(Mortierellomycota)、担子菌门(Basidiomycota);在属分类水平结构分析中仅有优势菌属2种,分别为Mortierella属和Tetracladium属。通过差异分析,发现翻地处理前后,不同土壤深度下真菌微生物群落组成发生较大变化。The soil in morchella cultivation field was used as experiment material.Using Illumina HiSeq high-throughput sequencing technology to analyze the soil fungal community structure,the changes of soil fungal community structure before and after soil tilting during morchella cultivation were studied,in order to provide a reference basis for the change of soil fungal community structure during morchella cultivation.The results showed that there were three dominant phyla in the four soil samples,namely Ascomycota,Mortierellomycota and Basidiomycota.There were only two dominant genera,Mortierella and Tetracladium.Through the difference analysis,it was found that the composition of fungal microbial community changed greatly at different soil depths before and after soil flipping.

关 键 词：羊肚菌 翻地处理 HiSeq高通量测序 真菌群落结构 

分 类 号：S646.9[农业科学—蔬菜学]