陇南花椒根腐病病原菌的分离鉴定及生物学特性的研究  

Isolation,Identification and Biological Characteristics of the Pathogen Bacteria of Zanthoxylum bungeanum Root Rot in Longnan

作　　者：田凤鸣[1] 陈强[1] 王瀚[1] 卓平清 孙杰[1] TIAN Fengming;CHEN Qiang;WANG Han;ZHUO Pingqing;SUN Jie(College of Life Science,Longnan Teachers College/Center for Research and Development of Longnan Characteristic Agro-Bioresource,Longnan 742500,China)

机构地区：[1]陇南师范高等专科学校农林技术学院/陇南特色农业生物资源研究开发中心,甘肃陇南742500

出　　处：《东北农业科学》2022年第1期95-99,107,共6页Journal of Northeast Agricultural Sciences

基　　金：2019年陇南市科技指导性计划项目(2019-ZD-13、2019-ZD-17);甘肃省高等学校创新能力提升项目(2019A-189)。

摘　　要：本研究采用组织分离法、柯赫氏法则、结合形态学和真菌rDNA-ITS序列分析法分离鉴定出引起甘肃陇南花椒根腐病的主要病原菌,同时采用菌丝生长速率法研究不同光照、pH值、温度、培养基、碳源、氮源条件下病原菌生物学特性的测定,为后期病害的诊断和防治提供理论基础。结果表明:造成陇南花椒根腐病的主要致病菌为茄病镰刀菌(Fusarium solani),光照条件对菌落的生长无明显差异,适宜菌落生长的pH值为5~7,最适温度为25~30℃,最适培养基为PDA,葡萄糖和乳糖为最佳碳源,硝酸钠为最佳氮源。In this study,tissue isolation method,Koch’s rule,combined morphology and fungal rDNA-ITS sequence analysis method were used to isolate and identify the main pathogens causing Zanthoxylum bungeanum root rot in Gansu Longnan.At the same time,the mycelium growth rate method was used to determine the effect of different light,pH,temperature,medium,carbon source and nitrogen source on the growth of pathogenic bacteria,providing a theoretical basis for the diagnosis and control of later diseases.The results show that the main pathogen causing the root rot of Longnan Zanthoxylum bungeanum is Fusarium solani.There is no obvious difference in the growth of the colony under the light condition.The pH value of suitable colony growth is 5-7,the optimum temperature is 25-30℃,the optimum medium is PDA,glucose and lactose are the best carbon source,and sodium nitrate is the best nitrogen source.Therefore,the main pathogens and the biological characteristics of the pathogens of Longnan Zanthoxylum bungeanum root rot are clarified,which provides a theoretical basis for the later study of the pathogenic mechanism and comprehensive control of the root rot of Zanthoxylum bungeanum.

关 键 词：花椒 根腐病 病原鉴定 茄病镰刀菌 

分 类 号：Q936[生物学—微生物学]