红菜薹茎基部分枝性状的遗传及内源激素影响因素研究  

Study on the Heredity of Stem Basal Branching and the Influence Factors of Endogenous Hormones in Purple Flowering Stalks

作　　者：余晨 别里扎特·努勒沙合提 沙爱华[1] 万正杰[2] YU Chen;BELIZAT Nureshahti;SHA Aihua;WAN Zhengjie(College of Agriculture,Yangtze University,Jingzhou 434000;College of Horticulture and Forestry Sciences,Huazhong Agricultural University/Key Laboratory of Horticultural Plant Biology(HZAU),MOE,Wuhan 430070,China)

机构地区：[1]长江大学农学院,湖北荆州434000 [2]华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室,武汉430070

出　　处：《东北农业科学》2022年第1期127-131,共5页Journal of Northeast Agricultural Sciences

基　　金：国家重点研发计划(2017YFD0101803)。

摘　　要：红菜薹茎基部分枝性状是薹用白菜的一种重要产量性状,内源激素对植物分枝过程有很重要的影响,为了探究与分枝相关的三种激素(生长素、细胞分裂素、独脚金内酯)对菜薹茎基部分枝性状的影响,本实验以红菜薹和菜心自交系为研究材料,构建六世代的遗传群体进行茎基部分枝性状的遗传分析,并且利用酶联免疫法(ELISA)测定三种激素含量,结果表明:红菜薹的茎基部分枝性状对无分枝性状为显性,F2分离群体的茎基部分枝性状表现连续分布,为数量性状,不符合典型的孟德尔遗传定律;两个亲本间生长素和细胞分裂素无显著差异,独脚金内酯含量差异较明显,结合两个亲本之间的表型差异分析,独脚金内酯可能是影响红菜薹茎基部分枝的直接因素,上述结果为红菜薹茎基部分枝的遗传克隆和形成机理研究奠定基础。The branching at the base of stem of purple flowering stalks is an essential yield trait of Brassica campestris L.The endogenous hormones have important effects on the branching process.In order to investigate the effects of three hormones(auxin,cytokinin and Strigolactone)related to branching on stem base of flowering stalks,the six generations of genetic groups were constructed to analyze the inheritance of some branch characters of stem base by using the enzyme linked immunosorbent(ELISA).The results showed that the characters of stem base branch were dominant to the characters without branch,and the characters of stem base branch of F2 segregation population were continuously distributed,which were quantitative characters and did not conform to the typical Mendelian inheritance law.There was no significant difference in auxin and cytokinin between the two parents,but there was a significant difference in the content of Strigolactone.Combined with the phenotypic difference analysis between the two parents,it was suggested that the Strigolactone might be the direct factor affecting the basal stem branching of purple flowering stalks.These results lay a foundation for genetic cloning and formation mechanism of stem basal branches of purple flowering stalks.

关 键 词：红菜薹 遗传分析 茎基部分枝 内源激素 

分 类 号：S634.9[农业科学—蔬菜学]