疏肝解郁汤对大鼠急性乳腺炎的抑制效果与机制研究  

Study on the inhibition effect and mechanism of Shugan Jieyu Decoction on rats with acute mastitis

作　　者：马晓佳 杨璐妍 黄冬涵 李本发 黄敏 MA Xiaojia;YANG Luyan;HUANG Donghan;LI Benfa;HUANG Min(Yunnan Provincial Hospital of Traditional Chinese Medicine/The First Affi liated Hospital of Yunnan University of Chinese Medicine,Kunming 650000,China;Yunnan University of Chinese Medicine,Kunming 650000,China)

机构地区：[1]云南省中医医院/云南中医药大学第一附属医院,昆明650000 [2]云南中医药大学,昆明650000

出　　处：《吉林中医药》2022年第2期208-212,共5页Jilin Journal of Chinese Medicine

基　　金：云南省教育厅科学研究基金项目(2019J1062);云南中医药大学-云南中医药大学第一附属医院联合基金项目(XYLH2019001)。

摘　　要：目的探讨疏肝解郁汤对乳腺炎大鼠的影响。方法疏肝解郁汤由瓜蒌,炒柴胡,牛蒡子,重楼,蒲公英,制香附,炒青皮,郁金,陈皮,玫瑰花,丝瓜络,鹿角霜混合制成水煎液。将SD大鼠分为空白组、模型组、疏肝解郁汤低、中、高剂量组(2.5、5、10 mg/kg),每组10只。造模前实验组灌胃汤剂,空白组与模型组灌胃生理盐水,每天1次,5 d后复制大鼠LPS乳腺炎模型,造模后12 h再给药1次。造模24 h后HE染色观察乳腺组织的病理学变化;Elisa检测大鼠血清与乳腺组织中IL-1、IL-6、TNF-α含量;Western Blot检测乳腺组织中p38、p-p38、MAPKAPK2、p-MAPKAPK2蛋白表达水平。结果模型组乳腺组织损伤严重,血清与组织中IL-1、IL-6、TNF-α的含量升高,组织中p-p38与p-MAPKAPK2蛋白表达水平升高;实验组乳腺组织损伤减轻,血清与组织中IL-1、IL-6、TNF-α的含量降低,组织中p-p38与p-MAPKAPK2蛋白表达水平降低(均P<0.05)。结论疏肝解郁汤能减轻大鼠急性乳腺炎症,降低血清与组织中的炎症因子,作用机制可能是抑制p38/MAPKAPK2通路。Objective To investigate the effect of Shugan Jieyu Decoction on rats with mastitis.Methods SD rats were divided into a blank group,a model group,and the Shugan Jieyu Decoction low-,medium-and high-dose groups (2.5,5 and 10 mg/kg,respectively),with 10 rats in each group.Before modeling,the three experimental groups were given Shugan Jieyu Decoction by gavage,while the blank and model groups were treated with normal saline by gavage,all once a day.After 5 days,the rat LPS mastitis model was replicated,and the drug was administered once again 12 hours after modeling.The pathological changes of breast tissues were observed by HE staining 24 hours after modeling.The contents of IL-1,IL-6 and TNF-α in rat serum and breast tissues were detected by Elisa.The protein expression levels of p38,p-p38,MAPKAPK2 and p-MAPKAPK2 in breast tissues were detected by Western Blot.Results The breast tissues in the model group were seriously damaged,and the levels of IL-1,IL-6 and TNF-α in serum and tissues were significantly increased,and the protein expression levels of p-p38 and p-MAPKAPK2 in tissues were increased.In the experimental group,the damage of breast tissues was reduced,and the levels of IL-1,IL-6 and TNF-α in serum and tissues were decreased,and the protein expression levels of p-p38 and p-MAPKAPK2 in tissues were decreased (all P<0.05).Conclusion Shugan Jieyu Decoction can relieve acute mastitis,and reduce inflammatory factors in serum and tissues,possibly by inhibiting the p38 /MAPKAPK2 pathway.

关 键 词：乳腺炎 疏肝解郁汤 中药 大鼠 P38MAPK 

分 类 号：R285.5[医药卫生—中药学]