黄甲软肝颗粒对人血白蛋白致免疫性肝纤维化模型大鼠的影响  

Protective Effect of Huangjia Ruangan Granules Against Immune Hepatic Fibrosis Induced by Human Serum Albumin in Rats

作　　者：高晓新 朱春玥 张茜茜 王佳帅 曹春然 胡宇驰 李雪梅 LIU Hongyan GAO Xiaoxin;ZHU Chunyue;ZHANG Qianqian;WANG Jiashuai;CAO Chunran;HU Yuchi;LIU Hongyan;LI Xuemei(Beijing Institute for Drug Control,NMPA Key Laboratory for Safety Research and Evaluation of Innovative Drug,Beijing Key Laboratory of Analysis and Evaluation on Chinese Medicine,Beijing 102206,China)

机构地区：[1]北京市药品检验研究院,国家药品监督管理局创新药物安全研究与评价重点实验室,中药成分分析与生物评价北京市重点实验室,北京102206 [2]不详

出　　处：《实验动物科学》2021年第6期28-32,共5页Laboratory Animal Science

摘　　要：目的观察黄甲软肝颗粒对人血白蛋白(human serum albumin,HSA)所致的Wistar大鼠免疫性肝纤维化的保护作用。方法采用体内实验,将72只雄性大鼠随机分成阴性对照组、模型组、阳性药组、黄甲软肝高、中以及低剂量组,建立HSA致大鼠免疫性肝纤维化模型,计算肝、脾系数,测定血清中肝纤维化标志物含量、丙氨酸氨基酸转移酶(ALT)、天门冬氨酸转移酶(AST)等相关生化指标,并检查肝脏病理形态改变。结果与模型组比较,黄甲软肝三个剂量组大鼠血清透明质酸(HA)含量显著降低;高剂量组血清层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)水平、ALT和AST显著降低;高剂量组及中剂量组肝纤维化病变明显减轻。结论黄甲软肝颗粒能显著改善HSA所致的免疫性肝纤维化大鼠的肝纤维化程度。Objective This study was designed to observe the protective effect of Huangjia Ruangan granules against immune hepatic fibrosis induced by human serum albumin( HSA) in rats. Method By vivo experiment,72 male rats were randomly divided into the normal group,the model group,the positive group,the high-dose group,the moderate-does group and the low-dose group of Huangjia Ruangan granules,n = 12. The rat model of immune hepatic fibrosis induced by HSA was established. The effects were evaluated by liver and spleen index,liver fibrosis markers,serum biochemical index and liver histopathological examination. Result The content of HA in 3 groups of Huangjia Ruangan was significantly lower than this in the model group,the levels of LN,PC Ⅲ,the contents of ALT and AST in the high-dose group were significantly lower than those in the model group. The degrees of liver fibrosis in the high-dose group and the moderate-dose group were significantly improved comparing with the model group. Conclusion Huangjia Ruangan granules can significantly improve hepatic fibrosis in rats induced by HSA.

关 键 词：黄甲软肝颗粒 人血白蛋白 免疫性肝纤维化 肝纤维化 大鼠 

分 类 号：Q95-33[生物学—动物学]