两种国产与一种进口小鼠肝炎病毒ELISA抗体检测试剂盒的比较研究  

Comparison of Two Domestic and One Imported ELISA Kits for the Detection of Mouse Hepatitis Virus Antibodies

作　　者：方姣 梁锦宁 方茜 朱康立 黄海娜 罗秋琳 潘炫孝 郭晓萍 FANG Jiao;LIANG Jinning;FANG Xi;ZHU Kangli;HUANG Haina;LUO Qiulin;PAN Xuanxiao;GUO Xiaoping(Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学,南宁530021

出　　处：《实验动物科学》2021年第6期33-36,共4页Laboratory Animal Science

基　　金：中央引导地方科技发展专项资金项目(桂科ZY20198024);广西医科大学创新训练计划项目(201910598416)。

摘　　要：目的本研究拟对比小鼠肝炎病毒(MHV)抗体ELISA检测试剂盒的敏感性、特异性、精密性和可信度,以筛选出既经济又可靠的检测试剂盒。方法选择两种国产与一种进口的ELISA试剂盒检测MHV抗体和以下5种病毒阳性血清:小鼠仙台病毒(SV)、小鼠肺炎病毒(PVM)、小鼠细小病毒(MVM)、小鼠鼠痘病毒(MPV)及小鼠呼肠孤Ⅲ型病毒(Reo-3),并进行敏感性、特异性、精密性及可信度实验比较。结果 ELISA国产B试剂盒的敏感性是国产A试剂盒的400倍,进口试剂盒的敏感性是国产B试剂盒的4倍;特异性实验显示国产A试剂盒与MPV无交叉反应,但与其他4种病毒阳性血清均有交叉反应,国产B试剂盒和进口试剂盒与MPV以外的5种病毒阳性血清均无交叉反应;精密性实验显示进口试剂盒批内平均变异系数为5.667%,国产A和国产B试剂盒批内平均变异系数分别为13.825%和13.827%;可信度实验显示国产B试剂盒和进口试剂盒的阳性和阴性检测符合率均为100%,国产A试剂盒的阳性和阴性检测符合率分别为40%和80%。结论 ELISA检测国产B试剂盒除敏感性和精密度低于检测的进口试剂盒外,其特异性和可信度均良好,国产A试剂盒的敏感性、特异性、精密性和可信度均低于检测的进口试剂盒。Objective To compare the sensitivity,specificity,precision and reliability of 2 domestic and 1 imported ELISA kits for the detection of mouse hepatitis virus( MHV) antibody,so as to screen out a kind of economic and reliable ELISA kit. Method Three kinds of ELISA kits were chosen to detect MHV antibodies and other 5 kinds of viruses such as Sendai virus( SV),Peneumonia virus of mice( PVM),Minute virus of mice( MVM),Poxvirus of mice( MPV) and Reovirus type Ⅲ( Reo-3),and compared their sensitivity,specificity,precision and reliability.Result There were significant differences in the sensitivity of the 3 kinds of kits. Domestic kit B is 400 times as sensitivity as domestic kit A,and the imported kit is 4 times sensitivity of kit B. There were no cross reaction with SV,PVM,MVM,MPV and Reo-3 in domestic kit B and the imported one,but there were cross reactions with domestic kit A. The precision test showed that the average coefficient of variation in the imported kit was 5. 667%,and in the domestic kits A and B were 13. 825% and 13. 827%. The reliability test showed that the coincidence rates of positive and negative tests of domestic kit B and the imported one were both 100%,and the coincidence rates of positive and negative tests of domestic kit A were 40% and 80%,respectively. Conclusion The specficity and reliability of domestic kit B are consistent with the improted one,but with lower sensitivity and precison than it. Domestic kit A is lower in all tests than others.

关 键 词：ELISA检测试剂盒 比较研究 小鼠肝炎病毒 

分 类 号：Q95-33[生物学—动物学]