4种柳树抗虫性和代谢组分析  

Insect resistance and metabolome of four willow species

作　　者：李文杰 崔露露 麻芸娇 梁海永[1] LI Wenjie;CUI Lulu;MA Yunjiao;LIANG Haiyong(College of Forestry,Hebei Agricultural University,Baoding 071000,Hebei,China)

机构地区：[1]河北农业大学林学院,河北保定071000

出　　处：《浙江农林大学学报》2022年第1期153-158,共6页Journal of Zhejiang A&F University

基　　金：河北省科技厅资助项目(17226320D-4)。

摘　　要：【目的】研究不同柳树Salix spp.对柳蓝叶甲Plagiodera versicolora的抗虫性,为选育抗虫性强的柳树品种提供参考。【方法】对旱柳S.matsudana、黄花柳S.caprea、垂柳S.babylonica以及三蕊柳S.triandra的3个无性系进行室内饲虫试验,并对抗虫组与非抗虫组进行差异次生代谢产物的测定与筛选。【结果】各无性系室内饲虫成活率旱柳为98%、垂柳为93%、黄花柳为90%,三蕊柳的3个无性系均为0。非抗虫组与抗虫组有23种主要的差异代谢产物,其中阿魏酰胍丁胺、抗坏血酸、没食子酸、绿茶儿茶酚、没食子儿茶素、杨梅苷、葡萄酸铜、D-(-)-阿拉伯糖、4-羟基-3-甲氧基肉桂酸乙酯、N-阿魏酰胍丁胺、葡糖酸、2′,4′,5,7-四羟基异黄酮、N-乙酰-DL-色氨酸、异泽兰黄素3-葡萄糖苷、十六聚甲烷基鞘氨醇等15种产物表现为上调,而槲皮素-3-O-(6″-O-乙酰基)-β-D-吡喃半乳糖苷、橙皮甙、芦丁、聚半乳糖醛酸、2,5-二羟基苯甲酸、槲皮素O-乙酰己烯苷、3-羟基-5-甲基苯酚-1-氧化基-β-D-葡萄糖、槲皮素等8种产物表现为下调。【结论】三蕊柳对柳蓝叶甲的抗虫性最佳;非抗虫组与抗虫组之间的主要次生代谢产物存在显著差异,表明三蕊柳的次生代谢产物可能会对柳蓝叶甲幼虫产生毒害作用,或缺乏幼虫生长发育所必需的营养物质而导致柳蓝叶甲幼虫死亡。图4表1参23。[Objective]This purpose is to explore the insect resistance of different willows(Salix spp.)to Plagiodera versicolora,and to provide reference for the breeding of willow cultivars with strong insect resistance.[Method]S.caprea,S.matsudana,S.babylonica and 3 clones of S.triandra were tested in laboratory for insect feeding,and the differential secondary metabolites between insect resistant and non insect resistant groups were determined and screened.[Result]The survival rate of indoor feeding was 98% for S.matsudana,93% for S.babylonica,90% for S.caprea and 0 for S.triandra.There were 23 main different metabolites between the non insect resistant group and the insect resistant group,among which 15 were upregulated,including fer-agmatine,L-ascorbic acid,gallic acid,(-)-epigallocatechin,(+)-gallocatechin,myricitrin,L-(+)-tartaric acid,D-(-)-arabinose,hydroxy-methoxycinnamate,N-feruloyl agmatine,gluconic acid,2′-Hydroxygenistein,N-Acetyl-DL-tryptophan,eupatilin 3-glucoside,and hexadecylsphingosine,and 8 were down-regulated,including quercetin 3-O-(6″-O-malonyl)-galactoside,hesperidin,rutin,D-galactopyranuronate,2,5-dihydroxy benzoic acid O-hexside,quercetin O-acetylhexoside,3-hydroxy-5-methylphenol-1-Oxy-β-Dglucose,and bioquercetin.[Conclusion]S.triandra has the best insect resistance to P.versicolora.There are significant differences in the main secondary metabolites between the non insect resistant group and the insect resistant group,which indicates that the secondary metabolites of S.triandra may be toxic to the larvae of P.versicolora,or lack of nutrients necessary for larvae growth and development,resulting in the death of the larvae.[Ch,4 fig.1 tab.23 ref.]

关 键 词：柳树 代谢组 次生代谢产物 柳蓝叶甲 

分 类 号：S722.3[农业科学—林木遗传育种]