正交实验优选桑籽中1-脱氧野尻霉素的提取工艺  

Optimization of extraction technology of 1-deoxynojirimycin from mulberry seeds by orthogonal test

作　　者：吕志强[1] 李乔[2] 闫海龙 徐文[1] 赵振寰[1] 巩丽丽[4] 田景振[4] LYU Zhiqiang;LI Qiao;YAN Hailong;XU Wen;ZHAO Zhenhuan;GONG Lili;TIAN Jingzhen(Pharmaceutical Department,the Affiliated Hospital of Qingdao University,Shangdong Province,Qingdao266000,China;Outpatient Department of General Affairs Department,Qingdao Vocational and Technical College of Hotel Management,Shangdong Province,Qingdao266000,China;Rehabilitation Department,Qingdao Women and Children′s Hospital,Shangdong Province,Qingdao266000,China;College of Pharmacy,Shandong University of TCM,Shangdong Province,Jin′an250355,China)

机构地区：[1]青岛大学附属医院药学部,山东青岛266000 [2]青岛酒店管理职业技术学院总务处门诊部,山东青岛266000 [3]青岛妇女儿童医院康复科,山东青岛266000 [4]山东中医药大学药学院,山东济南250355

出　　处：《中国当代医药》2022年第4期45-49,共5页China Modern Medicine

基　　金：山东省高等学校科技计划项目(J12LM58)。

摘　　要：目的探讨正交实验优选桑籽中1-脱氧野尻霉素(DNJ)的提取工艺。方法采用柱前衍生—高效液相色谱法,以桑籽DNJ提取率为指标,在单因素实验的基础上,分别考察了浸提温度、乙醇浓度、盐酸浓度等因素对桑籽DNJ提取率的影响。进行了工艺优化的正交实验,确定了桑籽DNJ提取的最佳工艺。结果对提取时间、溶剂量、提取次数等进行单因素实验考察分析,差异无统计学意义(P>0.05);进一步对盐酸浓度、乙醇浓度及浸提温度进行正交实验方差分析,盐酸浓度对DNJ提取效果影响显著,差异有统计学意义(P<0.05)。综合考虑各因素确定最佳提取方案为:盐酸浓度0.05 mol/L、乙醇浓度50%、浸提温度70℃。在此条件下,桑籽中DNJ的提取率为0.59%。结论在盐酸浓度0.05 mol/L、乙醇浓度50%、浸提温度70℃的条件下桑籽中DNJ的提取率较高,该方法简单易行,可作为桑籽中DNJ的提取工艺。Objective To optimize the extraction process of 1-deoxynojirimycin(DNJ)from mulberry seeds by orthogonal test.Methods The extraction rate of DNJ from mulberry seeds was determined by precolumn derivatization high performance liquid chromatography(HPLC).Based on single factor experiment,the effects of extraction temperature,ethanol concentration and hydrochloric acid concentration on the extraction rate of DNJ from mulberry seeds were investigated.The optimum extraction process of DNJ from mulberry seed was determined by orthogonal test.Results Single factor experiment was conducted to investigate and analyze the extraction time,solvent amount,extraction times,etc.,the differences were not statistically significant(P>0.05).The orthogonal experimental variance analysis of hydrochloric acid concentration,ethanol concentration and extraction temperature showed that the hydrochloric acid concentration had significant effect on the extraction effect of DNJ,the difference was statistically significant(P<0.05).Considering all factors,the best extraction scheme is determined as follows:hydrochloric acid concentration 0.05 mol/L,ethanol concentration 50%,extraction temperature 70℃.Under these conditions,the extraction rate of DNJ was 0.59%.Conclusion Under the conditions of hydrochloric acid concentration of 0.05 mol/L,ethanol concentration of 50%and extraction temperature of 70℃,the extraction rate of DNJ from mulberry seeds is the highest.This method is simple and can be used as the extraction process of DNJ from mulberry seeds.

关 键 词：桑籽 1-脱氧野尻霉素 正交实验 反相高效液相色谱法 柱前衍生化 

分 类 号：R284[医药卫生—中药学]