霍乱弧菌新发菌株中vipA,vipB和clpV对T6SS转录调控  

Transcriptional Regulation of T6SS by vipA,vipB and clpV in the New Pandemic Vibrio cholerae Strain

作　　者：吴文轩 郭扬 张幸鼎 赵文婧 WU Wen-xuan;GUO Yang;ZHANG Xing-ding;ZHAO Wen-jing(School of Medcine,Sun Yat-sen University,Shenzhen 518107,China)

机构地区：[1]中山大学医学院,广东深圳518107

出　　处：《中山大学学报（医学科学版）》2022年第1期77-85,共9页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(3190010294)。

摘　　要：【目的】探索霍乱弧菌新发菌株H1的六型分泌系统(T6SS)存在的转录调控机制。【方法】本研究以转录组测序为基础,分析H1株分别由于vipA敲除(H1ΔvipA),vipB敲除(H1ΔvipB)和clpV敲除(H1ΔclpV)时T6SS基因簇的转录水平,并对其差异基因进行了基因本体论(GO)富集和京都基因与基因组百科全书(KEGG)富集。【结果】发现H1ΔvipA有22个差异基因(FDR<0.01,|log_(2)FC|≥1),其中有18个属于T6SS基因簇;并且除vipA外,其转录水平均呈上升趋势。结合GO富集和KEGG富集结果,发现vipA的缺失会引起与霍乱弧菌群体感应相关基因(hapR)的转录水平显著降低。【结论】我们得出结论 vipA基因在霍乱弧菌H1菌中是一个具有多重功能的基因,一是编码T6SS鞘结构蛋白,二是参与T6SS调控;推测hapR可能参与H1株中vipA对T6SS基因簇的转录调控。【Objective】To explore the transcriptional control mechanism of type Ⅵ secretion system(T6SS)in the new strain of Vibrio cholerae H1.【Methods】This study was based on transcriptome analyzed the transcription level of T6SS gene clusters of H1 strain separately knockout vipA(H1ΔvipA),vipB(H1ΔvipB)and clpV(H1ΔclpV),and carried out Gene ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment of the differential genes.【Results】It was found that there were 22 differential genes(FDR<0.01,|log_(2)FC|≥1)in H1ΔvipA,18 of which belong to the T6SS gene cluster,and besides vipA,the average transcribed level showed an upward trend. Combined with GO enrichment and KEGG enrichment results,it was found that the absence of vipA decreased the transcription levels of the quorum sensing gene(hapR).【Conclusions】We concluded that the vipA gene is a multi-functional gene in Vibrio cholerae H1,one is encoded T6 SS structure protein and the other is taken apart in T6SS regulation,and we speculate hapR may have a role to play in the transcription regulation of T6SS gene clusters by vipA in H1 strain.

关 键 词：六型分泌系统 霍乱弧菌 转录调控 

分 类 号：Q78[生物学—分子生物学]