不同频率电针对内淋巴积水豚鼠耳蜗形态及血浆cAMP、AQP2的影响  

Effects of Electroacupuncture of Different Frequencies on the Morphology of Cochlea and Plasma cAMP and AQP2 in Guinea Pig Models of Endolymphatic Hydrops

作　　者：蒋丽元[1] 史莹莺 叶恬恬 JIANG Liyuan;SHI Yingying;YE Tiantian(Hangzhou Hospital of Traditional Chinese Medicine,Hangzhou 310007,China;Hangzhou Dingqiao Hospital,Hangzhou 310022,China;Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]杭州市中医院,杭州310007 [2]杭州市丁桥医院,杭州310022 [3]浙江中医药大学,杭州310053

出　　处：《上海针灸杂志》2022年第2期191-197,共7页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金项目(81704153)。

摘　　要：目的观察不同频率电针刺激百会穴和听宫穴对梅尼埃病内淋巴积水(EH)模型豚鼠耳蜗积水程度、耳蜗螺旋神经节超微结构及血浆环磷酸腺苷(cAMP)、水通道蛋白2(AQP2)浓度变化的影响,探究电针治疗EH的适宜频率,并分析其关键起效机制,为临床运用针灸治疗梅尼埃病提供实验基础。方法将健康雄性豚鼠随机分为空白组、模型组、针刺组、2 Hz电针组、100 Hz电针组,其中每组8只用于检测耳蜗积水程度、血浆cAMP、AQP2浓度变化;另外每组5只用于检测耳蜗螺旋神经节超微结构变化。采用腹腔注射醋酸去氨加压素(DDAVP)制作EH模型。2 Hz电针组造模后采用频率为2 Hz的电针刺激百会穴及一侧听宫穴,留针20 min,每日1次,连续10 d;100 Hz电针组选用频率100 Hz的电针,其余操作同2 Hz电针组。针刺组予针刺百会及一侧听宫穴,但不通电。5组豚鼠均于干预结束后检测耳蜗积水程度,并计算中阶面积/(中阶面积+前庭阶面积)占比,检测血浆cAMP、AQP2浓度,检测耳蜗螺旋神经节超微结构变化。结果与空白组比较,模型组豚鼠耳蜗积水程度增加,螺旋神经节细胞变性损伤,耳蜗中阶面积/(中阶面积+前庭阶面积)、cAMP、AQP2浓度增加(P<0.01);与模型组比较,各治疗组豚鼠耳蜗积水程度得到不同程度减轻,中阶面积/(中阶面积+前庭阶面积)显著降低(P<0.01),螺旋神经节细胞变性损伤得到改善;100 Hz电针对EH模型豚鼠耳蜗积水程度改善及螺旋神经节细胞修复作用更明显,且100 Hz电针能有效抑制EH模型豚鼠血浆cAMP、AQP2的过表达(P<0.01)。结论低频(2 Hz)与高频(100 Hz)电针治疗均能降低EH模型豚鼠耳蜗积水,其中高频治疗组效果更优,其机制可能依赖于对血浆cAMP、AQP2水平的良性调整,抑制细胞变性损伤,促进细胞修复。Objective To observe the effects of stimulating Baihui(GV20)and Tinggong(SI19)with electroacupuncture(EA)of different frequencies on hydrops in the cochlea,the ultrastructure of the spiral ganglion,and the contents of plasma cyclic adenosine monophosphate(cAMP)and aquaporin-2(AQP2)in guinea pig models of Meniere’s disease(MD)with endolymphatic hydrops(EH),for discovering the optimal EA frequency in treating EH,analyzing its critical action mechanism,and providing the experimental foundation for acupuncture-moxibustion treatment of MD.Method Healthy male guinea pigs were randomly divided into a blank control group,a model group,an acupuncture group,a 2 Hz EA group,and a 100 Hz EA group.Eight animals in each group were used to detect the hydrops degree in the cochlea and the contents of plasma cAMP and AQP2.The other five animals in each group were used to observe the changes in the ultrastructure of the cochlear spiral ganglion.The EH model was established using intraperitoneal injection of 1-deamino-8-D-arginine vasopressin(DDAVP).After modeling,the 2 Hz EA group received 2 Hz EA stimulation to Baihui and unilateral Tinggong acupoints,with the needles retained for 20 min once daily for 10 consecutive days;the 100 Hz EA group received 100 Hz EA stimulation with the same operation as in the 2 Hz EA group.The acupuncture group only received acupuncture at Baihui and unilateral Tinggong acupoints without electrical stimulation.The five groups of guinea pigs all underwent the examination of hydrops in the cochlea to calculate the ratio of scala media area to(scala media area+scala vestibule area),the detection of the contents of plasma cAMP and AQP2,and observation of the changes of the ultrastructure of the cochlear spiral ganglion.Result Compared with the blank control group,the cochlear hydrops was more serious,the spiral ganglion cells showed degenerative damage,and the ratio of scala media area to(scala media area+scala vestibule area)and the contents of cAMP and AQP2 increased in the model group(P<0.01);compar

关 键 词：电针 电针频率 梅尼埃病 内淋巴积水 环磷酸腺苷 水通道蛋白2 豚鼠 

分 类 号：R2-03[医药卫生—中医学]