4种脾虚造模法的横向对比研究  

A comparative study of four types of spleen deficiency modeling method

作　　者：王启航 陆瑞敏 陈萌 张冬梅[2] WANG Qihang;LU Ruimin;CHEN Meng;ZHANG Dongmei(Chinese Medicine College,Beijing University of Chinese Medicine,Beijing 100029,China;Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京中医药大学中医学院,北京100029 [2]北京中医药大学东直门医院,北京100700

出　　处：《天津中医药》2022年第2期238-243,共6页Tianjin Journal of Traditional Chinese Medicine

基　　金：北京中医药新奥奖励基金课题项目(2017-XAJLJJ-004)。

摘　　要：[目的]通过对4种常见脾虚小鼠造模方法进行横向对比研究,评价各造模法脾虚损伤程度及其适用性。[方法]100只小鼠随机分为空白对照组(去离子水每只每天0.5 mL)、苦寒泻下组(100%番泻叶水浸液每只每日0.5 mL)、饮食失节组(喂饲甘蓝并每2日加喂猪脂1次)、劳倦过度组(每日捆尾力竭游泳1次)、利血平组(1%利血平,每日2 mL/kg,皮下注射),每组20只。持续造模10 d后,每组随机抽取10只取材,做相关指标检测,10只自然恢复10 d,通过对宏观体征、进食量、体质量及游泳时长的测定,观察各组宏观指标的稳定性。计算各组胸腺、脾脏指数,血液分析仪检测血液学指标,比色法测定血清D-木糖水平,碘-淀粉酶比色法测定血清淀粉酶活性,实时荧光定量PCR法检测相对mtDNA拷贝数及线粒体自噬相关蛋白Pink1、Parkin mRNA的表达,对各组小鼠进行线粒体功能评价。[结果]与空白对照组相比:1)苦寒泻下组、饮食失节组,利血平组宏观指标表现差异明显(P<0.05),以利血平组最为显著,指标稳定性以苦寒泻下组显著(P<0.05),差异均具有统计学意义。2)苦寒泻下组、劳倦过度组、利血平组胸腺指数明显降低(P<0.05),差异具有统计学意义,劳倦过度组变化显著;苦寒泻下组与利血平组脾脏指数明显降低(P<0.05),差异具有统计学意义,利血平组变化显著。3)利血平组和苦寒泻下组红细胞(RBC)、白细胞(WBC)、血红蛋白(HGB)下降明显,差异有统计学意义(P<0.05)。4)各模型组小鼠血清D-木糖水平均降低(P<0.05),差异具有统计学意义。5)苦寒泻下组、饮食失节组、利血平组血清淀粉酶活性均有所降低(P<0.05),差异具有统计学意义,利血平组最为显著。6)苦寒泻下组、饮食失节组、劳倦过度组mtDNA拷贝数均有所降低(P<0.05),差异具有统计学意义,饮食失节组最为显著;苦寒泻下组和饮食失节组线粒体自噬相关蛋白Pink1 mRNA转录水平均�[Objective]Through the horizontal contrast study of four common models of spleen deficiency mice,to evaluate the damage degree and applicability of each method.[Methods]The 100 mice were randomly divided into control group(deionized water 0.5 mL/per day),bitter cold diarrhea group(100%senna leaf water extract 0.5 mL/per day),diet loss group(fed with cabbage and lard once every 2 days),fatigue group(tie the tail and swim exhausted)and reserpine group(1%reserpine for 2 mL/kg/day,subcutaneous injection)with 20 rats in each group.Ten samples were randomly selected from each group after 10 d of continuous modeling,and 10 samples were recovered naturally 10 d.The stability of macro indexes was observed by measuring macro signs,intake,weight and swimming time.The thymus and spleen indices of each group were calculated,hematological indexes were detected by blood analyzer,serum D-xylose level was determined by colorimetric method,serum amylase activity was determined by iodine-amylase colorimetric method,and the expression of relative mtDNA copy number and mitochondrial autophagy related protein Pink1,Parkin mRNA were detected by real-time fluorescence quantitative PCR method.mitochondrial function was evaluated in each group of mice.[Results]Compared with the control group:1)There were significant differences in macro indexes in the bitter cold diarrhea group,diet loss group and reserpine group(P<0.05),the most significant in the reserpine group and the significant stability in the lower group(P<0.05).2)Thymic index was decreased significantly in the bitter cold diarrhea group,fatigue group and reserpine group(P<0.05),the most significant in the reserpine group(P<0.05),and spleen index was decreased significantly in the bitter cold diarrhea group and reserpine group(P<0.05),the most significant in the reserpine group.3)The RBC,WBC,HGB of reserpine group and bitter cold diarrhea group were decreased significantly(P<0.05).4)The serum D-xylan water was decreased on average in each model group(P<0.05).5)Serum amylase activi

关 键 词：苦寒泻下造模法 饮食失节造模法 劳倦过度造模法 利血平造模法 横向研究 

分 类 号：R256.3[医药卫生—中医内科学]