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作 者:仇玉燕 张志勇[2] 陈淑吟[2] 倪可雯 贾超峰[1,2] 孟乾 祝斐[2] 张志伟[2] 汤晓建 QIU Yuyan;ZHANG Zhiyong;CHEN Shuyin;NI Kewen;JIA Chaofeng;MENG Qian;ZHU Fei;ZHANG Zhiwei;TANG Xiaojian(Shanghai Ocean University/National Demonstration Center for Experimental Fisheries Science Education,Shanghai 201306,China;Marine Fisheries Research Institute of Jiangsu Province,Nantong 226007,China)
机构地区:[1]上海海洋大学/水产科学国家级实验教学示范中心,上海201306 [2]江苏省海洋水产研究所,江苏南通226007
出 处:《南方水产科学》2022年第1期59-67,共9页South China Fisheries Science
基 金:江苏省农业重大新品种创制项目(PZC201744);江苏省农业科技自主创新资金项目[CX(17)2021]。
摘 要:比较了黑鲷(Acanthopagrus schlegelii)♀×真鲷(Pagrus major)♂杂交子二代(杂交鲷或杂交F_(2))与黑鲷幼鱼在不同投喂频率下生长性能、生理生化指标的差异表现,为新品种选育提供基础依据。选用杂交F;及黑鲷幼鱼为研究对象,设4个投喂频率组(1T:1次·d^(-1)、2T:2次·d^(-1)、3T:3次·d^(-1)、4T:4次·d^(-1)),进行40 d的养殖实验。结果显示,投喂频率从1T升至2T,杂交F_(2)与黑鲷的增重率(WGR)、特定生长率(SGR)、成活率(SR)显著升高(P<0.05),胃蛋白酶、脂肪酶(LPS)、α-淀粉酶活力显著降低(P<0.05)。投喂频率超过2T时,杂交F_(2)和黑鲷各实验组的WGR、SGR、SR及消化酶活力均无显著差异(P>0.05);抗氧化酶活力3T与2T组间无显著差异(P>0.05),但显著高于4T(P<0.05)。相同的投喂频率下,2T组杂交F_(2)的WGR、SGR、胃蛋白酶、LPS均显著高于黑鲷(P<0.05),4T组杂交F_(2)的过氧化氢酶(CAT)、总超氧化物歧化酶(T-SOD)均显著高于黑鲷(P<0.05)。研究结果表明,杂交F_(2)及黑鲷幼鱼的适宜投喂频率为2T,且杂交F_(2)在所有组别中的生长性能表现均优于黑鲷。In order to study and compare the differences of growth performance,physiological and biochemical indexes between the hybrid porgy(HF_(2))of Acanthopagrus schlegelii♀(black porgy)×Pagrus major♂(red porgy)and A.schlegelii with different feeding frequencies,we selected the HF_(2)and A.schlegelii juveniles as the research objects,and set four feeding frequency groups:once·d^(-1),twice·d^(-1),3 times·d^(-1) and 4 times·d^(-1)(marked as 1 T,2 T,3 T and 4 T),and carried out a 40-day breeding experiment.The results show that when the feeding frequency increased from 1 T to 2 T,the weight gain rate(WGR),specific growth rate(SGR)and survival rate(SR)of the HF_(2)and A.schlegelii increased significantly(P<0.05),while the activities of pepsin,lipase andα-amylase decreased significantly(P<0.05).There was no significant difference in the WGR,SGR,SR and digestive enzyme activities among the HF_(2)and A.schlegelii groups(P>0.05).When the feeding frequency was more than 2 T,there was no significant difference in the WGR,SGR,SR and digestive enzyme activity among the experimental groups(P>0.05).The antioxidant enzyme activity had no significant difference between 3 T and 2T groups(P>0.05),but was significantly higher than that in 4 T group(P>0.05).In addition,with the same feeding frequency,the WGR,SGR,pepsin and lipase(LPS)of the HF_(2)in 2T group were significantly higher than those of A.schlegelii;the catalase(CAT)and total superoxide dismutase(T-SOD)of the HF_(2)in 4 T group were significantly higher than those of A.schlegelii(P<0.05).Therefore,the optimum feeding frequency of the HF_(2)and A.schlegelii juveniles is 2 T,and the growth performance of the HF_(2)is obviously better than that in A.schlegelii among all the experimental groups.
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