纳米氧化锌标记BTLA蛋白的应用研究  

Application of ZnO Nanoparticles in Labeling Prelamin A Protein

作　　者：张雪杨 刘小柳 杨心怡 谭晓君 刘昆 刘薇 袁康瑞 陈稚 吴都督 Zhang Xueyang;Liu Xiaoliu;Yang Xinyi;Tan Xiaojun;Liu Kun;Liu Wei;Yuan Kangrui;Chen Zhi;Wu Dudu(School of Pharmacy,Guangdong Medical University,Dongguan 523808;Medical Laboratory of Luohu People's Hospital in Shenzhen,Shenzhen 518000,China)

机构地区：[1]广东医科大学药学院,广东东莞523808 [2]中国深圳市深圳罗湖人民医院医学实验室,广东深圳518000

出　　处：《广东化工》2022年第4期48-50,共3页Guangdong Chemical Industry

基　　金：广东省自然科学基金(No.2018A030307003);广东省中医药局科研项目(No.20212100);东莞市科技技特派员项目(No.20201800500062);广东医科大学南海海洋生物医药资源研发公共服务平台开放式基金项目(No.2HC18013,No.2HC18016),广东医科大学科研基金项目(No.GDMUZ2020006);深圳市医疗卫生三名工程(No.SZSM201601062)。

摘　　要：通过氧化还原合成法制备了水溶性的ZnO量子点(w-ZnO),并考察了纳米氧化锌的浓度、孵育时间对HEK-293的细胞毒性。结果表明,合成的纳米氧化锌粒径约为12 nm,在浓度小于800μM,孵育时间小于12 h,对HEK-293细胞毒性较小。同时利用静电作用将链霉素亲和素连接到Zn O表面,再利用抗原和抗体的特异性结合以及树突状细胞(Dendritic cell,DC)对量子点的内吞作用对DC中B/T淋巴细胞弱化因子(BTLA)以及HVEM蛋白进行标记,从而考察DC抗结核的分子机制。The water-soluble ZnO quantum dots(w-ZnO) were prepared by redox synthesis, and the cytotoxicity of the concentration of nano-zinc oxide and the incubation time on HEK-293 was investigated. The results show that the synthesized nano-zinc oxide has a particle size of about 12 nm, at a concentration of less than 800 μM, and an incubation time of less than 12 h, which is less toxic to HEK-293 cells. At the same time, the electrostatic effect is used to connect streptavidin to the surface of ZnO, and then the specific binding of antigen and antibody and the endocytosis of quantum dots by dendritic cells(DC) can affect the B/T lymph in DC. Cell weakening factor(BTLA) and HVEM protein are labeled for labeling.

关 键 词：结核病 氧化锌 量子点 树突状细胞 淋巴细胞弱化因子 

分 类 号：R52[医药卫生—内科学] TB383.1[医药卫生—临床医学] O657.3[一般工业技术—材料科学与工程]