HPLC法同时测定骨碎补中两种黄酮类化合物含量  

Simultaneous Determination of Two Fla vonoids in Rhizoma Drynariae by HPLC

作　　者：陈雪 雷霞[2] 王迪[1] 薛傲 丁立新[1] 张宁[2] Chen Xue;Lei Xia;Wang Di;Xue Ao;Ding Lixin;Zhang Ning(Jiamusi University,Jiamusi 154007;Heilongjiang University of Chinese Medicine,Jiamusi 154007,China)

机构地区：[1]佳木斯大学,黑龙江佳木斯154007 [2]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007

出　　处：《广东化工》2022年第4期188-190,共3页Guangdong Chemical Industry

基　　金：黑龙江中医药大学基金项目2018RCD19;黑龙江省属高校科技成果研发、培育项目TSTAU-C2018020;黑龙江省自然科学基金项目,LH2020H097。

摘　　要：目的:研究骨碎补提取物中柚皮苷(Naringin,Nar)和新北美圣草苷(Neoeriocitrin,Neo)含量同时测定的方法。方法:采用CQ5-4069 ODs色谱柱,HPLC-DAD测定骨碎补提取物中两种黄酮类成分柚皮苷和新北美圣草苷含量。结果:骨碎补55%乙醇提取部位柚皮苷含量为32.19%,新北美圣草苷含量为17.66%。结论:高效液相色谱法(HPLC)可以同时测定骨碎补提取物中柚皮苷和新北美圣草苷的含量,可用于骨碎补提取物的质量控制。Objective: To study a method for the simultaneous determination of Naringin(Nar) and Neoeriocitrin(Neo) in Drynaria fortunei extract. Methods:CQ5-4069 ODs chromatographic column and HPLC-DAD were used to determine the content of two flavonoids(Nar and Neo) in Drynaria fortunei extract. Results:The content of Nar content in the 55 % ethanol extract of Rhizoma Drynariae was 32.19 %, and the content of Neo was 17.66 %. Conclusion: High performance liquid chromatography(HPLC) can simultaneously determine the contents of Nar and Neo in Rhizoma Rhizoma Drynariae extract and can be used for the quality control of Rhizoma Rhizoma Drynariae extract.

关 键 词：高效液相色谱法 骨碎补提取物 新北美圣草苷 柚皮苷 含量测定 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]