某矿井下作业人员外周血血浆miRNAs表达研究  

MiRNA expressions in peripheral blood plasma of workers in a certain mine

作　　者：王东方 齐雪松[1] 吴丽娜 高刚[1] 阮健磊[1] 朴春南[1] 刘建香[1] Wang Dongfang;Qi Xuesong;Wu Lina;Gao Gang;Ruan Jianlei;Piao Chunnan;Liu Jianxiang(Key Laboratory of Radiological Protection and Nuclear Emergency,China CDC,National Institute for Radiological Protection,Chinese Center for Disease Control and Prevention,Beijing 100088,China;National Center for Occupational Safety and Health,Beijing 102300,China)

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室,北京100088 [2]国家卫生健康委职业安全卫生研究中心,北京102300

出　　处：《中华放射医学与防护杂志》2022年第1期1-6,共6页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的探讨氡对矿井下作业人员外周血血浆miR-16、miR-106b、miR-449a、let-7g、miR-21、miR-221、miR-34a表达的影响。方法采用简单随机抽样法分别选取矿井下作业人员(井下组)46名和矿井上作业人员(对照组)38名。采用实时荧光定量PCR(qPCR)方法, 检测研究对象外周血血浆中miRNAs的相对表达水平。比较miRNAs在井下组和对照组外周血血浆中的差异表达, 分析miRNAs的差异性表达与累积剂量等因素的关系。结果井下组外周血血浆miR-106b、miR-21、miR-221明显高于对照组(Z=-2.32、-2.47、-2.79, P<0.05), 其倍数变化(Fc)分别为1.61、1.75、1.30;miR-16、miR-449a、let-7g、miR-34a高于对照组, 但差异无统计学意义(P>0.05)。控制年龄、体质量指数(BMI)、吸烟等因素后, miR-16、miR-106b、let-7g、miR-21、miR-221的差异性表达和氡暴露累积剂量呈正相关(t=2.50、3.31、2.60、2.95、3.25, P<0.05), 而miR-449a、miR-34a的表达改变与氡暴露累积剂量无显著相关(P>0.05)。结论 miR-106b、miR-21、miR-221可能具有作为氡致辐射损伤的早期标志物的潜在价值。Objective To investigate the effect of radon on the expressions of miR-16,miR-106b,miR-449a,let-7g,miR-21,miR-221 and miR-34a in peripheral blood plasma of miners.Methods A total of 46 randomly selected miners worked underground(the underground group)and 38 miners worked aboveground(the control group).MiRNA levels in the underground and control groups were detected by qRT-PCR and their relationship with cumulative effective dose was further analyzed.Results The levels of miR-106b,miR-21,miR-221 in plasma of the study group were significantly higher than those in the control group(Z=-2.32,-2.47,-2.79,P<0.05),the corresponding Fc values were 1.61,1.75,1.30,respectively.The levels of miR-16,miR-449a,let-7g and miR-34a were slightly higher than those in the control group(P>0.05).After controlling of confounding factors such as age,BMI and smoking,the alteration of miR-16,miR-106b,let-7g,miR-21 and miR-221 in plasma of the underground group were positively correlated with the cumulative effective dose(t=2.50,3.31,2.60,2.95,3.25,P<0.05).No significant difference was observed in the plasma levels of miR-449a and miR-34a between the two groups(P>0.05).Conclusions miR-106b,miR-21 and miR-221 could be used as potential biomarkers of radon exposure.

关 键 词：氡 矿井下作业人员 血浆 MIRNAS 

分 类 号：R131[医药卫生—劳动卫生]