高效液相色谱法同时测定功劳木药材中9种化学成分含量  

Simultaneous Determination of Nine Compounds in Mahoniae Caulis by HPLC

作　　者：黄洋 HUANG Yang(School of Pharmaceutical Sciences,Guangzhou Medical University·Key Laboratory of Molecular Target&Clinical Pharmacology and the State Key Laboratory of Respiratory Disease,Guangzhou,Guangdong,China 511436)

机构地区：[1]广州医科大学药学院·广东省分子靶标与临床药理学重点实验室,广东广州511436

出　　处：《中国药业》2022年第4期79-82,共4页China Pharmaceuticals

基　　金：广东省深圳市科技计划项目[JCYJ20190806171605522];广东省广州市科技计划项目[202102020516]。

摘　　要：目的建立同时测定功劳木药材中9种化学成分含量的高效液相色谱法。方法色谱柱为Waters XBridge C_(18)柱(250 mm×4.6 mm,5μm);流动相为0.1%磷酸水溶液-乙腈(梯度洗脱),流速为1.0 mL/min,检测波长为210 nm,柱温为25℃,进样量为10μL。结果氯化木兰花碱、绿原酸、小檗胺、四氢药根碱、非洲防己碱、盐酸药根碱、粉防己碱、盐酸巴马汀、盐酸小檗碱质量浓度分别在0.04~81.06μg/mL(r=0.9999)、0.06~60.05μg/mL(r=0.9976)、0.02~10.44μg/mL(r=0.9985)、0.008~10.44μg/mL(r=0.9998)、0.05~49.67μg/mL(r=0.9999)、0.12~248.03μg/mL(r=0.9999)、0.42~39.18μg/mL(r=0.9999)、0.34~353.08μg/mL(r=0.9997)、0.29~295.53μg/mL(r=0.9999)范围内与峰面积线性关系良好;精密度、稳定性、重复性试验的RSD均小于3.0%(n=6);平均回收率分别为99.38%,99.17%,106.60%,99.58%,99.59%,99.72%,100.00%,99.58%,99.55%,RSD分别为0.28%,0.44%,1.86%,1.47%,0.68%,0.39%,0.87%,0.37%,0.32%(n=6)。结论该方法操作简便,专属性和重复性均良好,结果准确可靠,可用于同时测定功劳木药材中9种化学成分的含量。Objective To establish a high-performance liquid chromatography(HPLC)method for the simultaneous determination of nine compounds in Mahoniae Caulis.Methods The chromatographic column was Waters XBridge C_(18) column(250 mm×4.6 mm,5μm),the mobile phase was 0.1%phosphoric acid solution-acetonitrile(gradient elution),the flow rate was 1.0 mL/min,and the detection wavelength was 210 nm,the column temperature was 25℃,the injection volume was 10μL.Results The mass concentrations of magnoflorine chloride,chlorogenic acid,berbamine,corypalmine,columbamine,jatrorrhizine hydrochloride,tetrandrine,palmatine hydrochloride and berberine hydrochloride showed a good relationship with the peak area in the ranges of 0.04-81.06μg/mL(r=0.9999),0.06-60.05μg/mL(r=0.9976),0.02-10.44μg/mL(r=0.9985),0.008-10.44μg/mL(r=0.9998),0.05-49.67μg/mL(r=0.9999),0.12-248.03μg/mL(r=0.9999),0.42-39.18μg/mL(r=0.9999),0.34-353.08μg/mL(r=0.9997),0.29-295.53μg/mL(r=0.9999).The RSDs of precision,stability and repeatability tests were less than 3.0%(n=6).The average recoveries of the above nine compounds were 99.38%,99.17%,106.60%,99.58%,99.59%,99.72%,100.00%,99.58%,99.55%with RSDs of 0.28%,0.44%,1.86%,1.47%,0.68%,0.39%,0.87%,0.37%,0.32%(n=6),respectively.Conclusion The method is simple,specific,reproducible,accurate and reliable,which can be used for the simultaneous determination of nine compounds in Mahoniae Caulis.

关 键 词：高效液相色谱法 功劳木 化学成分 含量测定 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]