广东省2020年首次报道E基因型柯萨奇病毒B组3型的鉴定及基因特征分析  

Novel Analyses of the Genetic Characteristics and Identification of Genotype E Coxsackievirus B3 Strains in Guangdong Province,China,in 2020

作　　者：杨倩[1] 曾汉日[2] 郑焕英[2] 严冬梅[1] 祝双利[1] 宋洋[1] 王东艳[1] 韩振志 李冀琛 张勇[1] 许文波[1] YANG Qian;ZENG Hanri;ZHENG Huanying;YAN Dongmei;ZHU Shuangli;SONG Yang;WANG Dongyan;HAN Zhenzhi;LI Jichen;ZHANG Yong;XU Wenbo(WHO WPRO Regional Polio Reference Laboratory,National Health and Family Planning Commission Key Laboratory for Medical Virology,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Guangdong Center for Disease Control and Prevention,Guangzhou 511430,China;Department of Medical Microbiology,Weifang Medical University,Weifang 261053,China)

机构地区：[1]世界卫生组织西太平洋区脊髓灰质炎参比实验室国家卫生和健康委员会医学病毒学和病毒病重点实验室中国疾病预防控制中心病毒病预防控制所,北京102206 [2]广东省疾病预防控制中心,广州511430 [3]潍坊医学院医学微生物教研室,潍坊261053

出　　处：《病毒学报》2022年第1期57-63,共7页Chinese Journal of Virology

基　　金：十三五国家科技重大专项课题(项目号:2018ZX10713002),题目:病毒性传染病病原谱和病毒基因变异变迁规律研究;十三五国家科技重大专项课题(项目号:2018ZX10305409-004-002),题目:宏基因组学快速感染诊断产品和传染病原分类方法开发;北京市自然科学基金-海淀原始创新联合基金项目(L192014),题目:EV-A71疫苗大规模应用后对我国手足口病病原谱的影响及其保护效果评价研究。

摘　　要：柯萨奇病毒B组3型(Coxsackievirus B3,CVB3)是肠道病毒中流行较为广泛的血清型之一,在全球多个国家和地区曾报道儿童急性心肌炎、无菌性脑膜炎、手足口病等的暴发流行,严重威胁儿童健康和公共卫生安全。本研究对广东省2020年手足口病患者标本中分离到的8株CVB3进行基因特征和进化分析,结果显示分离到的8株CVB3 VP1区核苷酸序列相似性为98.2%~99.5%,与原型株Nancy的核苷酸序列相似性在77.9%~78.5%之间,与其他CVB3中国大陆流行株的核苷酸序列相似性为79.6%~82.2%。8株CVB3均为E基因型,为广东省首次报道。8株CVB3分离株在进化树上聚集,提示病毒发生了局部传播。全基因组序列分析提示2株广东CVB3分离株在非结构蛋白区有重组现象的发生。本研究为E基因型CVB3在我国的流行传播和疾病防控提供基础资料。Coxsackievirus B3(CVB3)is an important etiologic agent for acute myocarditis,aseptic meningitis as well as hand,foot,and mouth disease(HFMD).These diseases are serious threats to children and public health worldwide.To understand their evolutionary characteristics,we sequenced eight CVB3 strains isolated from HFMD samples in Guangdong Province(China).The eight CVB3 strains shared 98.2%–99.5%nucleotide sequence identity in the entire VP1 region.The nucleotide-sequence similarity of the VP1 region with the CVB3 prototype Nancy and other Chinese CVB3 strains was 77.9%–78.5%and 79.6%–82.2%,respectively.The eight CVB3 strains belonged to genotype E based on phylogenetic analyses,which we report for the first time for Guangdong Province.More attention should be paid to the possibility of local spread in Guangdong Province.Phylogenetic trees and Simplot analyses revealed frequent recombination in the nonstructural regions within EV-B.Our study provides basic data for genotype E CVB3 to aid disease control and prevention.

关 键 词：柯萨奇病毒B组3型 基因特征 手足口病 E基因型 

分 类 号：R373.2[医药卫生—病原生物学]