2018-2019年云南省部分地区鼠类携带汉坦病毒基因型别分析  

Genotyping of Hantaviruses from Rodents in Yunnan Province,China,2018-2019

作　　者：尚翠 杨丽芬 杜珊珊 Joseph O.Shaibu 周济华[2] 黄晓霞[1] 韩茜[2] 李川[1] 李阿茜 王芹[1] 梁米芳[1] 王世文[1] 李德新[1] 冯云[2] 李建东[1] SHANG Cui;YANG Lifen;DU Shanshan;Joseph OShaibu;ZHOU Jihua;HUANG Xiaoxia;HAN Qian;LI Chuan;LI Aqian;WANG Qin;LIANG Mifang;WANG Shiwen;LI Dexin;FENG Yun;LI Jiandong(Key Laboratory of Medical Virology,Ministry of Health,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Key Laboratory of Natural Epidemic Disease Prevention and Control Technology,Yunnan Institute of Endemic Diseases Control and Prevention,Dali 671000,China;Centre for Human Virology and Genomics,Nigerian Institute of Medical Research,Lagos 91000,Nigeria)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所卫生部医学病毒和病毒病重点实验室,北京102206 [2]云南省地方病防治所云南省自然疫源性疾病防控技术重点实验室,大理671000 [3]Centre for Human Virology and Genomics,Nigerian Institute of Medical Research,Lagos 91000

出　　处：《病毒学报》2022年第1期149-155,共7页Chinese Journal of Virology

基　　金：重大传染病防治专项(项目号:2016ZX10004222-002),题目:非洲病毒性出血热实验室检测及血清流行病学调查研究;重大传染病防治专项(项目号:2018ZX10711001),题目:病毒感染高通量快速检测与应急筛检技术研究;重大传染病防治专项(项目号:2017ZX10104001),题目:基于全基因组的病毒网络化监测和溯源技术体系研究。

摘　　要：为了解云南省汉坦病毒的分子流行病学特征,本研究在云南省祥云县、泸西县、禄丰县和楚雄市4个地区布点,采集鼠类动物标本,运用实时荧光RT-PCR检测鼠肺标本中的汉坦病毒中的汉滩病毒和首尔病毒核酸,通过PCR扩增S节段的全基因序列,并进行核苷酸同源性分析和遗传进化分析。结果共捕获鼠类动物70份,包含10个鼠种,其中褐家鼠30只,占42.9%,其次是鼩鼱12只,占17.1%;核酸检测阳性标本19份,均为首尔病毒,检出率为27.1%,源自祥云县的标本检出率在四个地区中最高(32.0%)。从鼠肺标本中扩增得到8条汉坦病毒S节段全基因序列,相似性为87.0%~99.7%,遗传进化分析显示基因序列聚集形成2个分支,一个分支属于S1亚型,另一个分支在遗传进化分析和blast比对分析中与其他亚型同源性均小于90%,属首尔病毒中独立的新的遗传进化分支,提示了云南省鼠类动物间多基因亚型首尔汉坦病毒流行,应进一步关注由宿主动物溢出感染人的潜力。To explore the molecular epidemiological characteristics of hantaviruses in Yunnan Province,China,rodents were collected from Xiangyun,Luxi,Lufeng and Chuxiong city.The genomic RNA of hantaviruses in the lung samples were detected by real-time fluorescence reverse transcription-polymerase chain reaction.The whole sequence of S fragments was obtained by overlapping PCR amplification.Seventy rodents belonging to 10 species were captured,of which Rattus norvegicus accounted for 42.9%,followed by shrews at 17.1%.The lung samples of 19 rodents were found to be positive for the genomic RNA of the Seoul virus;the detection rate was 27.1%(among which 52.6%was from Rattus norvegicus)and the samples from Xiangyun County had the highest detection rate among the four regions(32.0%).The full sequences of S fragments were amplified from the samples of eight rodents.Homology comparison showed that the similarity between these sequences was87.0%–99.7%.In the phylogenetic tree,the eight sequences were clustered into two clades.One clade belonged to the S1 subtype,and the other clade had<90%homology with other subtypes,thereby suggesting it was a new subtype.These results demonstrated the diversities of Seoul hantaviruses circulating among rodents in Yunnan Province.Attention should be paid to the potential“spillover”of viruses from host animals to humans.

关 键 词：汉坦病毒 首尔病毒 汉滩病毒 基因型 

分 类 号：R373.9[医药卫生—病原生物学]