Fas/FasL基因和蛋白表达对精子浓度和活力的影响  

Impacts of the expressions of Fas and FasL mRNA and proteins on sperm concentration and motility

作　　者：戴芳[1] 林泽森 买鹏宇 朱闽[4] DAI Fang;LIN Ze-sen;MAI Peng-yu;ZHU Min(Department of Laboratory,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning,Guangxi 530001,China;Guangxi University of Chinese Medicine,Nanning,Guangxi 530001,China;Department of Science and Technology,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning,Guangxi 530001,China;Department of Andrology,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning,Guangxi 530001,China)

机构地区：[1]广西中医药大学附属瑞康医院检验科,广西南宁530001 [2]广西中医药大学,广西南宁530001 [3]广西中医药大学附属瑞康医院科技科,广西南宁530001 [4]广西中医药大学附属瑞康医院男性科,广西南宁530001

出　　处：《中华男科学杂志》2021年第12期1069-1074,共6页National Journal of Andrology

基　　金：广西壮族自治区中医药管理局科研课题(GZZC2019112);广西高校壮医方药基础与应用研究重点实验室科研课题(桂教科研﹝2014﹞6号zyfy201505);广西中医药大学2019年研究生教育创新计划项目(YCSY20190057)。

摘　　要：目的:探讨精液中细胞凋亡Fas/FasL基因和蛋白的表达对精子浓度和活力的影响。方法:将精液标本根据精子浓度和前向运动精子百分率分为4组:正常精子组、弱精子症组、少精子症组和少弱精子症组,每组20例。分别检测各组精液常规参数、Fas/FasL蛋白浓度、Fas/FasL基因表达水平。结果:(1)4组精子的浓度(×10^(6)/ml)分别为68.11±35.49、92.21±60.96、8.55±2.82、5.96±3.80;4组精子的前向运动精子百分率(%)为:49.40±13.86、22.12±7.13、40.77±8.41、14.53±9.74;(2)ELISA法检测4组精子Fas蛋白水平(pg/ml)分别为:381.07±52.37、425.03±50.56、442.32±84.88、448.42±84.79,与正常组比较均偏高,有统计学差异(P<0.05),与精子浓度呈负相关关系(r=-0.377,P<0.01),与前向运动精子呈负相关关系(r=-0.350,P<0.01);Fasl蛋白水平(pg/ml)分别为:167.49±29.91、166.98±27.39、169.51±32.62、171.46±32.61,与正常组比较无统计学差异(P>0.05);(3)RT-PCR方法检测4组的Fas基因的相对定量结果分别是:1、0.88±1.17、2.55±2.11、0.69±0.90,与正常组比较,弱精子症组和少弱精子症组的Fas基因表达下调,精子活力较低的样本中Fas基因表达较低;FasL基因的结果分别是:1、1.99±1.81、2.08±2.06、2.03±2.23,与正常组比较,少精子症组和少弱精子症组FasL基因表达上调,精子浓度较低的样本FasL基因表达较高;与正常组比较,弱精子症组Fas、FasL表达均下调,少精子症组Fas、FasL表达均上调,而少弱精子症组Fas表达下调,FasL表达上调。前向运动精子(PR%)与Fas mRNA基因呈正相关关系(r=0.355,P=0.01);精子总活力(PR+NR%)与Fas mRNA基因呈正相关关系(r=0.358,P<0.01);精子浓度与FasL mRNA基因表达呈负相关关系(r=-0.305,P<0.05);其余指标无明显相关关系。结论:Fas/FasL在正常、弱精子、少精子和少弱精子症组的表达水平有差异,Fas/FasL表达上调可能促进了生精细胞和精子细胞的凋亡,这可能是少精子症的发生的Objective:To investigate the influence of the expressions of apoptosis-related Fas and FasL mRNA and proteins on sperm concentration and motility.Methods:We collected semen samples from 80 adult males and divided them into four groups of an equal number according to sperm concentration and the percentage of progressively motile sperm(PMS):normal,asthenospermia(AS),oligozoospermia(OS)and oligoasthenospermia(OAS).We examined the routine semen parameters,the levels of Fas and FasL proteins and the expressions of Fas and FasL genes in different groups.Results:The sperm concentrations in the normal,AS,OS and OAS groups were(68.11±35.49),(92.21±60.96),(8.55±2.82)and(5.96±3.80)×10^(6)/ml,respectively,and the percentages of PMS were(49.40±13.86)%,(22.12±7.13)%,(40.77±8.41)%and(14.53±9.74),respectively.The Fas protein level was significantly higher in the AS,OS and OAS than in the normal group([425.03±50.56],[442.32±84.88]and[448.42±84.79]vs[381.07±52.37]pg/ml,P<0.05),correlated negatively with sperm concentration(r=-0.377,P<0.01)and PMS(r=-0.350,P<0.01),but exhibited no statistically significant differences between the former three and latter group([166.98±27.39],[169.51±32.62]and[171.46±32.61]vs[167.49±29.91]pg/ml,P>0.05).The relative levels of the Fas gene in the normal,AS,OS and OAS groups were 1,(0.88±1.17),(2.55±2.11)and(0.69±0.90)respectively,lower in the AS and OAS than in the normal group,and positively correlated with sperm motility;those of the FasL gene were 1,(1.99±1.81),(2.08±2.06)and(2.03±2.23)respectively,higher in the OS and OAS than in the normal group,and negatively correlated with sperm motility.Compared with the normal group,the expressions of Fas and FasL were down-regulated in the AS but up-regulated in the OS group;the expression of Fas,however,was down-regulated and that of FasL up-regulated in the OAS group.The expression of Fas mRNA was positively correlated with the percentage of PMS(r=0.355,P=0.01)and total sperm motility(r=0.358,P<0.01),while sperm concentration negativ

关 键 词：精子 FAS FASL 精子浓度 精子活力 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]