戊吡虫胍原药对大鼠的亚慢性经口毒性  

The subchronic oral toxicity of guadipyr in SD rats

作　　者：卢昊[1,2] 黎炎梅 付少华[1,2] LU Hao;LI Yan-mei;FU Shao-hua(Hubei Center for Disease Control and Prevention,Wuhan 430079,China;Hubei Key Laboratory of Toxicology Applications,Wuhan 430074,China)

机构地区：[1]湖北省疾病预防控制中心,武汉430079 [2]应用毒理湖北省重点实验室,武汉430074

出　　处：《农药》2022年第2期110-113,126,共5页Agrochemicals

摘　　要：[目的]通过经口给予大鼠不同剂量的戊吡虫胍原药,研究戊吡虫胍原药对大鼠的亚慢性毒性作用。[方法]选取60~80 g的Wistar大鼠,共80只,按体质量随机分为阴性对照组及低、中、高剂量组,每组雌雄各10只。低、中、高剂量组分别为10、50、250 mg/kg b.w.,连续经口给予戊吡虫胍原药90 d,阴性对照组给予蒸馏水。[结果]试验期间动物未见明显的中毒症状和死亡。与阴性对照组比较:雌性高剂量组第6~8周平均体质量降低(P<0.05)。雌性中剂量组嗜酸性细胞升高(P<0.05),高剂量组网织红细胞、嗜酸性细胞均升高(P<0.01);雄鼠高剂量组的白细胞、嗜酸性细胞、网织红细胞升高(P<0.01、P<0.05、P<0.01)。雌性高剂量组总胆固醇(CHO)、血清胆碱酯酶(CHE)均升高(P<0.05),谷丙转氨酶(ALT)降低(P<0.01)。雄性中剂量组谷丙转氨酶(ALT)、甘油三脂(TG)均升高(P<0.05),高剂量组血糖(GLU)升高(P<0.05)。雌性高剂量组脾脏脏体比偏高(P<0.05);雄性中剂量组肝脏、脾脏、肾脏脏体比偏高(P<0.01、P<0.05、P<0.05),高剂量组肝脏、脾脏脏体比均偏高(P<0.01)。尿常规检查结果未见明显差异。组织病理学检查未见明显异常的组织病理学变化。[结论]在本试验条件下,戊吡虫胍原药大鼠3个月亚慢性经口毒性试验的无害作用剂量(NOAEL)为10 mg/kg b.w.,最小有害作用剂量(LOAEL)为50 mg/kg b.w.。[Aims]This study aims to conduct the 90-days oral toxicity of guadipyr in rats.[Methods]A total of 80 wistar rats(60-80 g)were randomly divided into negative control group,low,middle and high dose group according to body weight,10 male and 10 female in each group.The low,middle and high dose groups were 10,50,250 mg/kg b.w.,respectively.The negative control group was given the same volume of distilled water.[Results]Compared to the control group:The body weights of female rats in high dose group were significantly less than those of the groups during 6-8 weeks(P<0.05).The EO%were higher for female rats in middle and high dose group(P<0.05).The RET%and EO%were higher for female rats in high dose group(P<0.01).The WBC,EO%and RET%were higher for male rats in high dose group(P<0.01,P<0.05,P<0.01).The CHO%and CHE%were higher for female rats in high dose group(P<0.05).The ALT%were lower for female rats in high dose group(P<0.01).The ALT%and TG%were higher for male rats in middle dose group(P<0.05)and the GLU were higher for male rats in high dose group(P<0.05).The organ coefficients of spleen in the female rats of high dose group increased significantly(P<0.05).The organ coefficients of livers,spleen and kidney in the male rats of middle dose group increased significantly(P<0.01,P<0.05,P<0.05).The organ coefficients of livers and spleen in the male rats of high dose group increased significantly(P<0.01).There was no significant difference in statistical results.The histopathology showed no significant abnormal histopathology changes.[Conclusions]Based on this study,the NOAEL of subacute toxicity of guadipyr to rats by oral contamination is 10 mg/kg b.w.,the LOAEL of subacute toxicity of guadipyr to rats by oral contamination is 50 mg/kg b.w.

关 键 词：戊吡虫胍 亚慢性经口毒性 无害作用剂量(NOAEL) 最小有害作用剂量(LOAEL) 

分 类 号：TQ450.2[化学工程—农药化工]