部分还原氧化石墨烯的制备、表征及对人宫颈癌HeLa细胞的体外杀伤作用  

Preparation,Characterization of Partially Reduced Graphene Oxide and Its Killing Effect on Human Cervical Cancer Cells

作　　者：王雪丽 宋相伟 解颜宁 杜妮阳 王振新[3] WANG Xueli;SONG Xiangwei;XIE Yanning;DU Niyang;WANG Zhenxin(Grain College,Jilin Business and Technology College,Changchun 130507,China;Life Science of College,Changchun Normal University,Changchun 130032,China;Changchun Institute of Applied Chemistry,the Chinese Academy of Sciences,Changchun 130022,China)

机构地区：[1]吉林工商学院粮食学院,长春130507 [2]长春师范大学生命科学学院,长春130032 [3]中国科学院长春应用化学研究所,长春130022

出　　处：《高等学校化学学报》2022年第2期85-92,共8页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21475126,21775145);吉林省科技发展计划项目(批准号:20190304051YY);吉林省教育厅项目(批准号:JJKH20210879KJ);吉林工商学院项目(批准号:KB2019[001]);长春师范大学自然科学基金(批准号:长师大自然合字[2018]第12号)资助。

摘　　要：以氧化石墨烯(GO)为原料,利用温和方法制备了3种不同还原程度的部分还原氧化石墨烯pRGO_(1),pRGO_(2)和pRGO_(3)(pRGO_(1-3));利用傅里叶变换红外光谱(FTIR)、拉曼光谱(Raman)、X射线光电子能谱(XPS)、紫外-可见光谱(UV-Vis)、透射电子显微镜(TEM)和EDS能谱对其结构和形貌进行了表征.细胞实验结果表明,无激光照射下pRGO_(1-3)本身的细胞毒性较低;近红外(NIR)激光照射下pRGO_(1-3)通过光热和光毒性双重作用杀伤肿瘤细胞.实验结果显示了pRGO在肿瘤光热疗法和光动力疗法领域的应用潜力.Partially reduced grphene oxides(pRGO_(1)-pRGO_(3),pRGO_(1-3)) were synthesized using a mild reduction method by incubating GO suspension under alkaline condition at room tempeature.Structure and morphology of the as-synthesized pRGO_(1-3) nanocomposites were confirmed by means of FTIR,Raman,XPS,UV-Vis,transmission electron microscopy(TEM)and energy dispersive spectrometer(EDS).pRGO possesses synergistic photodynamic and photothermal effects on tumor cell upon near-infrared(NIR)light irradiation.Consequently,pRGO can be expected to be a new nano carrier for tumor photodynamic and photothermal therapy.

关 键 词：部分还原氧化石墨烯 宫颈癌细胞 光热治疗 光毒性治疗 

分 类 号：O656.4[理学—分析化学]