黄芪多糖对慢性心力衰竭大鼠心肌细胞能量代谢的影响  

Effects of Astragalus Polysaccharides on the Energy Metabolism of Cardiomyocytes in Rats with Chronic Heart Failure

作　　者：刘莉莉[1] 王国良[1] LIU Lili;WANG Guoliang(First Affiliated Hospital of Henan University,Kaifeng 475001,Henan,China)

机构地区：[1]河南大学第一附属医院,河南开封475001

出　　处：《中西医结合心脑血管病杂志》2022年第3期420-426,共7页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：河南省医学科技攻关计划项目(No.2018020314)。

摘　　要：目的研究黄芪多糖(APS)对慢性心力衰竭(CHF)大鼠心肌细胞能量代谢的影响,并探讨相关机制。方法取60只无特定病原体(SPF)级SD大鼠,随机选取10只仅做假手术,设为对照组,其余50只建立CHF模型。将建模成功的41只大鼠随机分为CHF组(10只)、挽回组(10只)、APS组(10只)、阳性药物组(11只)。APS组予APS 400 mg/kg灌胃;挽回组予APS 400 mg/kg灌胃,Compound C水溶液2 mL灌胃;阳性药物组予卡托普利10 mg/kg灌胃;对照组与CHF组予等量生理盐水灌胃。连续干预28 d。末次干预后4 h采用超声心动仪测定心功能指标;脱颈处死大鼠测定心肌三磷酸腺苷(ATP)含量;透射电镜观察心肌细胞线粒体结构;苏木精-伊红(HE)染色观察心肌组织病理变化;凋亡细胞原位末端转移标记法(TUNEL)染色观察心肌细胞凋亡情况;蛋白免疫印迹(Western Blot)法检测心肌组织5-单磷酸腺苷活化蛋白激酶(AMPK)、磷酸化腺苷酸活化蛋白激酶(p-AMPK)、过氧化物酶体增殖物激活受体共激活因子-1(PGC-1α)蛋白相对表达量。结果与CHF组比较,挽回组、APS组、阳性药物组左心室射血分数(LVEF)、左心室缩短分数(LVFS)均升高(P<0.05),左心室舒张末期内径(LVEDD)、左心室收缩末期内径(LVESD)均缩短(P<0.05),心肌组织ATP含量均升高(P<0.05);与挽回组比较,APS组、阳性药物组LVEF、LVFS均升高(P<0.05),LVEDD、LVESD均缩短(P<0.05),心肌组织ATP含量均升高(P<0.05)。挽回组、APS组、阳性药物组线粒体排列与大小变规则,数量逐渐增加,空泡变性及肿胀减轻,线粒体嵴增加,心肌横纹、润盘变清晰。挽回组、APS组、阳性药物组心肌细胞形态结构损伤有所减轻,心肌横纹较为清晰。与CHF组比较,挽回组、APS组、阳性药物组心肌细胞凋亡率均降低(P<0.001),PGC-1α蛋白、p-AMPK/AMPK均升高(P<0.05);与挽回组比较,APS组、阳性药物组心肌细胞凋亡率均降低(P<0.001),PGC-1α蛋白、p-AMPK/AMPK均升高(P<0.001)Objective To study the effects of astragalus polysaccharide(APS)on the energy metabolism of rats in cardiomyocytes with chronic heart failure(CHF),and explore the related mechanisms.Methods A total of 60 sprague-dawley(SD)rats without specific pathogen(SPF)were selected,and 10 of them were randomly selected as sham operation control group,and the remaining 50 were as CHF model.The 41 rats successfully modeled were randomly divided into CHF group(10 rats),recovery group(10 rats),APS group(10 rats),and positive drug group(11 rats).APS group was treated with 400 mg/kg APS by gavage.Recovery group was treated with 400 mg/kg APS and 2 mL Compound C aqueous solution by gavage.Positive drug group was treated with captopril 10 mg/kg by gavage.Control group and CHF group were treated with the same amount of normal saline by gavage.The intervention lasted for 28 days.Echocardiograph was used to measure cardiac function indexes after the last intervention.The content of adenosine triphosphate(ATP)in myocardium of rats were determined after neck dissection.Mitochondrial structure of myocardial cells were observed by transmission electron microscope.Hematoxylin-eosin(HE)staining was used to observe the pathological changes of myocardial tissue.TUNEL staining was used to observe the apoptosis of cardiomyocytes.The relative expression levels of 5-adenosine monophosphate-activated protein kinase(AMPK),and phosphorylated adenosine monophosphoric activated protein kinase(P-AMPK),and peroxisome proliferator-activated receptor coactivator-1α(PGC-1α)protein in myocardial tissue were detected by Western Blot.Results Compared with CHF group,left ventricular ejection fraction(LVEF)and left ventricular shortening fraction(LVFS)of the recovery group,APS group,and positive drug group were increased(P<0.05),left ventricular end diastolic diameter(LVEDD)and left ventricular end systolic diameter(LVESD)were shortened(P<0.05),the ATP content in myocardial tissue were increased(P<0.05).Compared with the recovery group,LVEF and LVFS of the APS

关 键 词：慢性心力衰竭 黄芪多糖 能量代谢 心肌细胞凋亡 实验研究 

分 类 号：R54[医药卫生—心血管疾病]