热应激对摩拉和尼里-拉菲水牛血液生化指标的影响  

Effects of Heat Stress on Blood Biochemical Indexes of Murrahand Nili-Ravi Buffaloes

作　　者：刘新勇 谭正准[2] 李辉[2] 黄健[2] 罗华 曾令湖 程隽如 钟华配 杨春艳[2] 王晓丽[1] 覃广胜[2] LIU Xinyong;TAN Zhengzhun;LI Hui;HUANG Jian;LUO Hua;ZENG Linghu;CHENG Juanru;ZHONG Huapei;YANG Chunyan;WANG Xiaoli;QIN Guangsheng(College of Animal Science and Technology,Guangxi University,Nanning 530005,China;Guangxi Buffalo Research Institute,Chinese Academy of Agricultural Sciences,Nanning 530001,China)

机构地区：[1]广西大学动物科学技术学院,南宁530005 [2]中国农业科学院广西壮族自治区水牛研究所,南宁530001

出　　处：《中国畜牧兽医》2022年第2期521-528,共8页China Animal Husbandry & Veterinary Medicine

基　　金：广西科技计划项目(桂科AD19259009);亚热带农业生物资源保护与利用国家重点实验室开放课题(SKLCUSA-b201814);广西水牛研究所重点实验室开放课题(19A0301);广西自然科学基金项目(2018GXNSFAA281020)。

摘　　要：【目的】试验旨在探究热应激条件下摩拉和尼里-拉菲水牛的血液生化指标变化规律。【方法】选取体况相近的健康摩拉和尼里-拉菲水牛各11头。试验期划分为热应激期和非应激期,并于各试验期分别进行4次采血,每次间隔1周。测定血清激素浓度、抗氧化指标和离子浓度等生化指标。【结果】摩拉水牛热应激期血清超氧化物歧化酶(SOD)活性及总胆固醇(CHOL)、丙二醛(MDA)、热休克蛋白70(HSP70)、钾离子(K^(+))、钠离子(Na^(+))、氯离子(Cl^(-))含量均显著高于非应激期(P<0.05),谷丙转氨酶(ALT)、谷草转氨酶(AST)、谷胱甘肽过氧化物酶(GSH-Px)活性及总抗氧化能力(T-AOC)、胰岛素样生长因子1(IGF-1)、皮质醇(CORT)、瘦素(LEP)、钙离子(Ca^(2+))含量均显著低于非应激期(P<0.05);尼里-拉菲水牛热应激期血清白蛋白(ALB)、CHOL水平及谷氨酰基转移酶(GGT)活性均显著高于非应激期(P<0.05),球蛋白(GLOB)含量显著低于非应激期(P<0.05)。在热应激期,摩拉水牛ALT活性及总蛋白(TP)、GLOB、CHOL、尿素氮(BUN)、甘油三酯(TG)含量均显著高于尼里-拉菲水牛(P<0.05),ALB、四碘甲腺原氨酸(T_(4))、K^(+)含量均显著低于尼里-拉菲水牛(P<0.05);在非应激期,摩拉水牛ALT活性、BUN、TG、葡萄糖(GLU)含量及T-AOC均显著高于尼里-拉菲水牛(P<0.05)。【结论】热应激降低了摩拉和尼里-拉菲水牛的内分泌激素水平和抗氧化能力并影响了机体电解质平衡,尼里-拉菲水牛的免疫功能也有一定下降;在热应激条件下,摩拉水牛表现出更强的清除自由基能力,尼里-拉菲水牛表现出更好的代谢、体温调节能力以及电解质代偿能力。【Objective】The purpose of this study was to explore the changes of blood biochemical indexes of Murrah and Nili-Ravi buffaloes under heat stress.【Method】11 Murrah and 11 Nili-Ravi buffaloes with similar physical conditions and healthy physique were selected.The experimental period was divided into heat stress period and non stress period,and four blood samples were taken in each experimental period,with an interval of one week.The blood samples were measured for biochemical indexes such as serum hormone concentration,antioxidant index and ion concentration.【Result】For Murrah buffalos,the activity/content of superoxide dismutase(SOD),total cholesterol(CHOL),malondialdehyde(MDA),heat shock protein 70(HSP70),potassium ion(K^(+)),sodium ion(Na^(+))and chloride ion(Cl^(-))during heat stress were significantly higher than those in non stress period(P<0.05),while the activity/content of alanine aminotransferase(ALT),aspartate aminotransferase(AST),insulin-like growth factor 1(IGF-1),cortisol(CORT),leptin(LEP),glutathione peroxidase(GSH-Px),total antioxidant capacity(T-AOC)and calcium ion(Ca2+)were significantly lower than those in non stress period(P<0.05).For Nili-Ravi buffaloes,the content/activity of albumin(ALB),CHOL and glutamyltransferase(GGT)in the heat stress period were significantly higher than those in the non stress period(P<0.05),and the content of globulin(GLOB)in the heat stress period was significantly lower than that in the non stress period(P<0.05).In heat stress period,the activity/content of ALT,total protein(TP),GLOB,CHOL,urea nitrogen(BUN)and triglyceride(TG)in Murrah buffaloes were significantly higher than those in Nili-Ravi buffaloes(P<0.05),and the contents of ALB,tetraiodothyronine(T_(4))and K^(+)in Nili-Ravi buffaloes were significantly higher than those in Murrah buffaloes(P<0.05).In non stress period,the contents of ALT,BUN,TG,glucose(GLU)and T-AOC of Murrah buffaloes were significantly higher than those of Nili-Ravi buffaloes(P<0.05).【Conclusion】Heat stress reduced the endo

关 键 词：摩拉水牛 尼里-拉菲水牛 热应激 生化指标 

分 类 号：Q494[生物学—生理学]