大豆GmSg-5基因的克隆、序列分析及表达分析  

Cloning,sequence analysis and expression analysis of GmSg-5 gene in soybean

作　　者：沈悦 谈美霞 范姝姝 高浩竣 武文娟 王敏[1] 杜维俊[1] 赵晋忠[2] 岳爱琴[1] Shen Yue;Tan Meixia;Fan Shushu;Gao Haojun;Wu Wenjuan;Wang Min;Du Weijun;Zhao Jinzhong;Yue Aiqin(College of Agronomy,Shanxi Agrucultural University,Taigu 030801,China;Department of Basic Sciences,Shanxi Agrucultural University,Taigu 030801,China)

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]山西农业大学基础部,山西太谷030801

出　　处：《山西农业大学学报（自然科学版）》2022年第1期10-18,共9页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：山西省重点研发计划重点项目(201903D211006-1);山西省自然科学基金(201901D111225);山西农业大学育种工程项目(YZGC096)。

摘　　要：[目的]GmSg-5是大豆A类皂苷合成途径的关键酶基因。对大豆GmSg-5基因进行克隆、生物信息学分析及基因表达模式分析,为今后深入研究A类大豆皂苷的合成及大豆品质改良提供参考。[方法]以晋遗30为试验材料,采用ExPASy-Protparam等软件对GmSg-5基因和蛋白质序列进行生物信息学分析,利用PlantCARE对GmSg-5基因启动子序列进行分析,qRT-PCR方法分析GmSg-5基因在根、茎、叶不同组织、籽粒不同发育时期、激素及非生物胁迫诱导的表达情况。[结果]GmSg-5基因CDS全长1536 bp,编码511个氨基酸,编码蛋白主要由HEME和CPR两个结构域构成,相对分子量为58.6 kD,等电点(pI)为8.95。qRT-PCR分析表明,GmSg-5基因在根中表达量最高;开花后50 d籽粒中GmSg-5基因表达量最高。MJ、ABA诱导根中GmSg-5基因的表达,抑制叶中该基因的表达,H2O2、PEG和NaCl胁迫诱导根和叶中GmSg-5基因的表达。[结论]大豆GmSg-5基因参与多种非生物胁迫应答,在大豆响应和抵制非生物胁迫及激素诱导过程中发挥重要的作用。[Objective]TThe GmSg-5 gene is a key enzyme gene in the synthetic pathway of soybean A saponins.Cloning,bioinformatic analysis and gene expression pattern analysis of soybean GmSg-5 will provide reference for further research on the synthesis of group A saponins and soybean quality improvement in the future.[Methods]Using Jinyi 30 as the study material,the GmSg-5 gene and protein sequence were analyzed by ExPASy-Protparam and other software.GmSg-5 gene promoter sequence was analyzed by PlantCARE.The expression of GmSg-5 gene in different tissues of roots,stems,leaves,different stages of grain development,hormones and abiotic stress-induced expression were analyzed by qRT-PCR.[Results]The coding DNA sequences(CDS)of GmSg-5 gene was 1536 bp in length and encoded 511 amino acids.The encoded protein was mainly consisted of HEME and CPR domain,with a relative molecular weight of 58.6 kD and an isoelectric point(pI)of8.95.The qRT-PCR analysis results showed that GmSg-5 gene expression was highest in roots and in grains 50 days after flowering.GmSg-5 gene expression was induced by MJ and ABA in the root but suppressed in leaves.GmSg-5 gene was induced by H2 O2,PEG and NaCl in the root and leaves.[Conclusion]The GmSg-5 gene was involved in multiple abiotic stress responses and played an important role in soybean response and resistance to abiotic stress and hormone induction.

关 键 词：大豆 GmSg-5 生物信息学分析 激素 非生物胁迫 

分 类 号：S529[农业科学—作物学]