兰州百合多糖片段对X射线诱导的小鼠骨髓有核细胞损伤的保护作用  

Protective effect of Lanzhou lily polysaccharide fragment on X-ray-induced injury of bone marrow nucleated cells in mice

作　　者：郭忠[1] 马建秀 马艳庆 宋雷[1] 汪晨净[1] 郭淼 夏溪 赵晋[1] GUO Zhong;MAJianxiu;MAYanqing;SONG Lei;WANG Chenjing;GUO Miao;XIA Xi;ZHAO Jin(Medical College of Northwest Minzu University,Laboratory of Environmental Ecology and Population Healthin Northwest Minority Regions of Northwest Minzu University,Lanzhou 730030,China)

机构地区：[1]西北民族大学医学部药理学教研室,西北民族大学西北民族地区环境生态与人群健康实验室,兰州730030

出　　处：《辐射研究与辐射工艺学报》2022年第1期56-62,共7页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金项目(81560508);国家级大学生创新创业训练计划项目(202110742054)资助。

摘　　要：研究兰州百合多糖片段(Lanzhou lily polysaccharide fragment,LLP)对X射线诱导的小鼠骨髓有核细胞(Bone marrow nucleated cells,BMNCs)损伤的辐射防护作用。采用超声辅助热水法提取并制备百合粗多糖,经超滤法、Sephadex G-150色谱等方法分离纯化后得到LLP,其平均分子量为8629.8 Da。建立小鼠急性放射性损伤模型,设立对照组、单纯照射组及低、中、高不同浓度LLP给药组,检测各组BMNCs数量、微核率、DNA损伤情况以及细胞凋亡和周期变化。结果表明:与对照组相比,照射组辐射损伤明显;与单纯照射组相比,LLP中、高剂量组BMNCs数量分别升高45.5%(p<0.01)、49.0%(p<0.01);低、中、高剂量组微核率分别降低7.2%(p<0.05)、33.5%(p<0.01)和59.9%(p<0.01);中、高剂量组彗星电泳尾矩和Olive尾矩分别降低51.2%(p<0.01)、58.2%(p<0.01)和54.0%(p<0.01)、60.5%(p<0.01);低、中、高剂量组γH2AX荧光强度分别降低19.7%(p<0.01)、46.8%(p<0.01)和61.0%(p<0.01);低、中、高剂量组凋亡率分别降低43.7%(p<0.01)、45.5%(p<0.01)和54.6%(p<0.01);中、高剂量组G0/G1期细胞比例明显降低(p<0.01),中、高剂量组S期细胞比例显著升高(p<0.01),高剂量组G2/M期细胞比例显著升高(p<0.01)。结果提示,LLP能减少BMNCs损伤,降低微核率以及降低X射线引起的DNA双链断裂,还对X射线引起的细胞凋亡有保护作用。可能对X射线诱导的小鼠BMNCs损伤具有保护作用。This study focused on the radioprotective effects of Lanzhou lily polysaccharide fragment(LLP)on murine bone marrow nucleated cells(BMNCs)subjected to X-ray-induced injury.The lily polysaccharide extract was prepared using the ultrasonic auxiliary hot water method,followed by purification using ultrafiltration and Sephadex G-150 chromatography.The average molecular weight of the extract was 8629.8 Da.Unlike the control and simple irradiation groups,the murine model of acute radioactive injury model was treated with different amounts of LLPs.Each group was evaluated for the number of BMNCs,micronucleus ratio,DNA injury,and cyclical changes.The results revealed increased injury in the simple irradiation group than in the LLP-treated group.The number of BMNCs increased by 45.5%(p<0.01)and 49.0%(p<0.01)in the medium-and high-dose groups,respectively.The micronucleus ratio reduced by 7.2%(p<0.05),33.5%(p<0.01),and 59.9%in the low-,medium-,and high-dose groups,respectively.The tail moment and olive tail moment of the medium-and high-dose groups reduced by 51.2%(p<0.01),58.2%(p<0.01),and 54.0%(p<0.01),and 60.5%(p<0.01),respectively.The fluorescence intensity ofγH 2 AX reduced by 19.7%(p<0.01),46.8%(p<0.01),and 61.0%(p<0.01)in the low,medium-,and high-dose groups,respectively.The apoptosis rate reduced by 43.7%(p<0.01),45.5%(p<0.01),and 54.6%(p<0.01)in the low,medium,and high-dose groups,respectively.The ratio of G 0/G 1 cells significantly decreased in the medium-and high-dose groups(p<0.01).The ratio of S-phase cells significantly increased in the medium-and high-dose groups(p<0.01),whereas the ratio of G 2/M phase cells significantly increased in the high dose group(p<0.01).Collectively,these results indicate that LLP extract is effective in alleviating X-ray-induced damage in BMNC,reduced micronucleus ratio,and protects against X-ray-induced double strand breaks(DSB)and apoptosis.Thus,LLP extract may play an important role radioprotection against X-rays-induced damage in BMNCs.

关 键 词：兰州百合多糖片段 小鼠 骨髓有核细胞 X射线 辐射防护 

分 类 号：R81[医药卫生—放射医学] TL71[医药卫生—临床医学]