化妆品中11种抗菌药物的高效液相色谱法检测  

Detection of Eleven Antimicrobial Agents in Cosmetics by High Performance Liquid Chromatography

作　　者：伍英英 岑立怡 黄佳颖 曾子君 周智明 WU Yingying;CEN Liyi;HUANG Jiaying;ZENG Zijun;ZHOU Zhiming(Guangdong Institute for Drug Control/NMPA Key Laboratory for Safety Risk Assesment of Cosmetics,Guangzhou 510663,Guangdong,China)

机构地区：[1]广东省药品检验所/国家药监局化妆品风险评估重点实验室,广东广州510663

出　　处：《香料香精化妆品》2022年第1期42-45,共4页Flavour Fragrance Cosmetics

摘　　要：建立了化妆品中11种抗菌药物的高效液相色谱检测方法。样品用甲醇+四氢呋喃(80+20)提取,C18色谱柱分离,检测波长为240 nm,二极管阵列检测器检测。各待测物的方法检出限为20~40 mg/kg,在5.0~100 mg/L的线性范围内,所得的回归方程均呈较好的线性关系,相关系数大于0.99,方法回收率为86.1%~114.2%,相对标准偏差为0.5%~4.3%。该方法准确快速,操作简便,适用于化妆品中抗菌类药物的含量测定。A detection method of 11 antibacterial drugs in cosmetics was established by high performance liquid chromatography.The sample was extracted with methanol+tetrahydrofuran(80+20),separated on a C18 chromatographic column with a detection wavelength of 240 nm,and detected by a diode array detector.The detection limit of the method for each analyte was 20-40 mg/kg,within the linear range of 5.0-100 mg/L,the regression equations obtained showed a good linear relationship,and the correlation coefficient was greater than 0.99.The method recovery rate was 86.1%-114.2%,and the relative standard deviation was 0.5%-4.3%.The method is accurate,fast and easy to operate,which is suitable for the determination of antibacterial drugs in cosmetics.

关 键 词：高效液相色谱法 抗菌类药物 化妆品 

分 类 号：O657.72[理学—分析化学] TQ658[理学—化学]