胰高血糖素样肽-1对新生乳鼠脑缺血再灌注损伤的影响  

Effect of glucagon-like peptide-1 on cerebral ischemic-reperfusion injury in neonatal rats

作　　者：李承燕 周璇 唐兰芬 莫波 陈银慧 陈巧媚 闫星羽 敖当 LI Chengyan;ZHOU Xuan;TANG Lanfen;MO Bo;CHEN Yinhui;CHEN Qiaomei;YAN Xingyu;AO Dang(Department of Pediatrics,Affiliated Hospital of Guangdong Medical University,Guangdong Province,Zhanjiang 524000,China;Department of Pediatrics,Sichuan Academy of Medical Sciences·Sichuan Provincial People’s Hospital,Sichuan Province,Chengdu 610000,China)

机构地区：[1]广东医科大学附属医院儿科,广东湛江524000 [2]四川省医学科学院·四川省人民医院儿科,四川成都610000

出　　处：《中国医药导报》2022年第2期4-7,12,共5页China Medical Herald

基　　金：广东省基础与应用基础研究基金项目(2019A1515110564);广东省医学科学技术研究基金项目(A2020286)。

摘　　要：目的研究胰高血糖素样肽-1(GLP-1)对新生乳鼠脑缺血再灌注损伤的神经保护作用及机制。方法将18只7日龄SD乳鼠分为对照组、模型组及治疗组,每组6只。乳鼠麻醉后,解剖颈部暴露左颈总动脉,对照组仅暴露左颈总动脉,模型组及治疗组用血管夹夹闭左颈总动脉60 min后恢复血流。手术后治疗组给予利拉鲁肽注射液腹腔注射,对照组及模型组给予等量生理盐水腹腔注射。Longa法对乳鼠进行神经行为障碍评分;TUNEL法检测海马神经元凋亡细胞数;免疫组织化学法检测海马核因子κB(NF-κB)阳性细胞数;Western blot法检测海马组织NF-κB、肿瘤坏死因子-α(TNF-α)、诱导型一氧化氮合酶(iNOS)、Caspase-3蛋白的表达。结果模型组神经行为障碍评分、海马神经元凋亡细胞数、NF-κB阳性细胞数及TNF-α、iNOS、Caspase-3蛋白水平高于对照组,治疗组神经行为障碍评分、海马神经元凋亡细胞数、NF-κB阳性细胞数及TNF-α、iNOS、Caspase-3蛋白水平低于模型组,差异均有统计学意义(P<0.05)。模型组细胞核p-NF-κB蛋白水平高于细胞质NF-κB蛋白水平;治疗组细胞核p-NF-κB蛋白水平低于细胞质NF-κB蛋白水平,差异均有统计学意义(P<0.05)。结论GLP-1可能通过抑制NF-κB介导的炎症反应来减少缺血再灌注乳鼠海马神经元的细胞凋亡。Objective To study the neuroprotective effect and mechanism of glucagon-like peptide-1(GLP-1)on cerebral ischemia-reperfusion injury in neonatal rats.Methods Eighteen 7-day-old SD neonatal rats were divided into control group,model group,and treatment group,with six rats in each group.After anesthesia,the left common carotid artery was dissected in the neck of neonatal rats,while only the left common carotid artery was exposed in the control group,the blood flow of model group and treatment group was recovered after clipping the left common carotid artery for 60 min.After operation,treatment group was given Liraglutide Injection intraperitoneal injection,and control group and model group were given the same amount of normal saline intraperitoneal injection.Longa method was used to score neurobehavioral disorder in neonatal rats;TUNEL assay was used to detect the number of apoptotic cells in hippocampal neurons;the number of nuclear factorκB(NF-κB)positive cells in hippocampus was detected by immunohistochemistry;the expressions of NF-κB,tumor necrosis factor-α(TNF-α),inducible nitric oxide synthase(iNOS),and Caspase-3 protein in hippocampus tissues were detected by Western blot.Results The neurobehavioral disorder score,the number of apoptotic cells in hippocampal neurons,the number of NF-κB positive cells,and the levels of TNF-α,iNOS,and Caspase-3 protein in model group were higher than those in control group,the neurobehavioral disorder score,the number of apoptotic cells in hippocampal neurons,the number of NF-κB positive cells,and the levels of TNF-α,i NOS,and Caspase-3 protein in treatment group were lower than those in model group,and the differences were statistically significant(P<0.05).In model group,the level of intranuclear p-NF-κB protein level was higher than that in cytoplasmic NF-κB protein level;and in treatment group,the intranuclear p-NF-κB p65 protein level was lower than the cytoplasmic NF-κB protein level,and the differences were statistically significant(P<0.05).Conclusion GLP-1

关 键 词：胰高血糖素样肽-1 缺氧再灌注损伤 核因子ΚB 细胞凋亡 

分 类 号：R722.12[医药卫生—儿科]