不同浓度黄芩苷对高毒力肺炎克雷伯菌生长及生物被膜形成能力影响的初步探讨  

A preliminary study on the effects of different concentrations of baicalin on the growth and biofilm formation of hypervirulent Klebsiella pneumoniae

作　　者：韩佳慧 刘唐娟[1] 罗劲[1] 黄海珠 陈一强[1] HAN Jia-hui;LIU Tang-juan;LUO Jin;HUANG Hai-zhu;CHEN Yi-qiang(The First Affiliated Hospital of Guangxi Medical University,Nanning,Guangxi 530021,China)

机构地区：[1]广西医科大学第一附属医院,广西南宁530021

出　　处：《临床肺科杂志》2022年第3期342-345,共4页Journal of Clinical Pulmonary Medicine

基　　金：国家自然科学基金资助项目(No.81260002);广西高校中青年教师科研基础能力提升资助项目(No.2019KY0125);广西壮族自治区卫生健康委员会自筹经费科研课题资助(No.Z20200825)。

摘　　要：目的研究黄芩苷对高毒力肺炎克雷伯菌体外生长及生物被膜形成能力的影响。方法以编号为3的高毒力肺炎克雷伯菌临床菌株作为试验菌株,微量稀释法测定黄芩苷最低抑菌浓度(MIC);通过细菌生长曲线观察黄芩苷对高毒力肺炎克雷伯菌生长的影响;连续稀释法检测黄芩苷对高毒力肺炎克雷伯菌生物被膜内活菌计数的影响;结晶紫染色法半定量测定黄芩苷对高毒力肺炎克雷伯菌生物被膜生物量的影响;结果黄芩苷对高毒力肺炎克雷伯菌的MIC为2048μg/mL;结晶紫染色法半定量生物被膜形成示:终浓度分别为2048μg/mL、1024μg/mL、512μg/mL的黄芩苷试验组生物被膜形成明显少于空白对照组(P<0.05);生物被膜内活菌计数示:与空白对照组相比,终浓度分别为2048μg/mL、1024μg/mL、512μg/mL的黄芩苷试验组活菌计数均减少(P<0.05)。结论黄芩苷可抑制高毒力肺炎克雷伯菌生长及生物被膜形成。Objective To investigate the effects of baicalin on the in vitro growth and biofilm formation of hypervirulent Klebsiella pneumoniae.Methods Hypervirulent Klebsiella pneumoniae-3 obtained from clinical strain was selected as the study strain.The minimal inhibitory concentration(MIC)was measured by using a microdilution method;the bacterial growth curve was used to observe the effect of baicalin on the growth of hypervirulent Klebsiella pneumoniae;serial dilution method was used to assess the effect of baicalin on the count of viable bacteria in the biofilm of K.pneumoniae;semi-quantitative crystal violet staining method was used to assess the impact on biofilm biomass.Results The MIC of baicalin against hypervirulent Klebsiella pneumoniae was 2048μg/mL.The semi-quantitative results of crystal violet staining indicated that the biofilm formation of the baicalin test group with final concentrations of 2048μg/mL,1024μg/mL,and 512μg/mL was significantly less than that of the blank control group(P<0.05).The count of viable bacteria in the biofilm showed that compared with the blank control group,the viable counts of the baicalin test group with final concentrations of 2048μg/mL,1024μg/mL,and 512μg/mL were all reduced(P<0.05).Conclusion Baicalin can inhibitthe growth and biofilm formationof hypervirulent Klebsiella pneumoniae in vitro.

关 键 词：高毒力肺炎克雷伯菌 生物被膜 黄芩苷 

分 类 号：R446.5[医药卫生—诊断学]