Seed dormancy,germination and storage behavior of Magnolia sinica,a plant species with extremely small populations of Magnoliaceae  

作　　者：Liang Lin Lei Cai Lei Fan Jun-Chao Ma Xiang-Yun Yang Xiao-Jian Hu 

机构地区：[1]The Germplasm Bank of Wild Species,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming,650201,Yunnan,China [2]Yunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small Populations,Key Laboratory far Plant Diversity and Biogeography of East Asia,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming,650201,Yunnan,China

出　　处：《Plant Diversity》2022年第1期94-100,共7页植物多样性（英文版）

摘　　要：Magnolia sinica is one of the most endangered Magnoliaceae species in China.Seed biology information concerning its long-term ex situ conservation and utilization is insufficient.This study investigated dormancy status,germination requirements and storage behavior of M.sinica.Freshly matured seeds germinated to ca.86.5%at 25/15℃but poorly at 30℃;GA3 and moist chilling promoted germination significantly at 20℃.Embryos grew at temperatures(alternating or constant)between 20℃and 25℃,but not at 5℃or 30℃.Our results indicate that M.sinica seeds possibly have non-deep simple mor-phophysiological dormancy(MPD).Seeds survived desiccation to 9.27%and 4.85%moisture content(MC)as well as a further 6-month storage at-20℃and in liquid nitrogen,including recovery in vitro as excised embryos.The established protocol ensured that at least 58%of seedlings were obtained after both cold storage and cryopreservation.These results indicate that both conventional seed banking and cryopreservation have potential as long-term ex situ conservation methods,although further optimized approaches are recommended for this critically endangered magnolia species.

关 键 词：Magnolia sinica Seed Dormancy Storage behavior CRYOPRESERVATION Excised embryo 

分 类 号：Q945[生物学—植物学]