Structural basis of ALMT1-mediated aluminum resistance in Arabidopsis  

作　　者：Jiangqin Wang Xiafei Yu Zhong Jie Ding Xiaokang Zhang Yanping Luo Ximing Xu Yuan Xie Xiaoxiao Li Tian Yuan Shao Jian Zheng Wei Yang Jiangtao Guo 

机构地区：[1]Department of Biophysics,and Department of Pathology of Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou,Zhejiang,China [2]Department of Biophysics and Department of Neurosurgery,The First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou,Zhejiang,China [3]State Key Laboratory of Plant Physiology and Biochemistry,College of Life Sciences,Zhejiang University,Hangzhou,Zhejiang,China [4]Interdisciplinary Center for Brain Information,The Brain Cognition and Brain Disease Institute,Faculty of Life and Health Sciences,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,Guangdong,China [5]ShenzhenHong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions,Shenzhen,Guangdong,China [6]Key Laboratory of Marine Drugs of Ministry of Education,School of Medicine and Pharmacy,Ocean University of China,Qingdao,Shandong,China [7]Department of Cardiology,Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou,Zhejiang,China [8]Cancer Center,Zhejiang University,Hangzhou,Zhejiang,China [9]Liangzhu Laboratory,Zhejiang University Medical Center,1369 West Wenyi Road,Hangzhou,Zhejiang,China

出　　处：《Cell Research》2022年第1期89-98,共10页细胞研究（英文版）

基　　金：supported in part by the Ministry of Science and Technology(2020YFA0908501 and 2018YFA0508100 to J.G.,2016YFA0501100 to X.Z.);the National Natural Science Foundation of China(31870724 to J.G.,82030108,31872796 and 81571127 to W.Y.,31730006 to S.J.Z.,and 31600606 to X.Z.);National Major Special Project on New Drug Innovation of China(2018ZX09711001-004-005 to W.Y.);Zhejiang Provincial Natural Science Foundation(LR19C050002 to J.G.,LR16H090001 to W.Y.);the Fundamental Research Funds for the Central Universities(2021FZZX001-28 to J.G.and S.J.Z.);supported by MOE Frontier Science Center for Brain Science&Brain-Machine Integration,Zhejiang University;supported by Guangdong Provincial Key Laboratory of Brain Connectome and Behavior(2017B030301017);CAS Key Laboratory of Brain Connectome and Manipulation(2019DP173024).

摘　　要：The plant aluminum(Al)-activated malate transporter ALMT1 mediates the efflux of malate to chelate the Al in acidic soils and underlies the plant Al resistance.Here we present cryo-electron microscopy(cryo-EM)structures of Arabidopsis thaliana ALMT1(AtALMT1)in the apo,malate-bound,and Al-bound states at neutral and/or acidic pH at up to 3.0Åresolution.The AtALMT1 dimer assembles an anion channel and each subunit contains six transmembrane helices(TMs)and six cytosolicα-helices.Two pairs of Arg residues are located in the center of the channel pore and contribute to malate recognition.Al binds at the extracellular side of AtALMT1 and induces conformational changes of the TM1-2 loop and the TM5-6 loop,resulting in the opening of the extracellular gate.These structures,along with electrophysiological measurements,molecular dynamic simulations,and mutagenesis study in Arabidopsis,elucidate the structural basis for Al-activated malate transport by ALMT1.

关 键 词：ARABIDOPSIS RESISTANCE ACIDIC 

分 类 号：Q94[生物学—植物学]